Summary: Zymogram patterns from 84 strains of dermatophyte fungi were obtained using polyacrylamide gradient gel electrophoresis of total cell protein extracts. The enzymes investigated were α-naphthyl acetate esterase, acid phosphatase, lactate dehydrogenase, malate dehydrogenase, tetrazolium oxidase and catalase. These patterns were used to construct similarity matrices and dendrograms using computerized techniques. The results showed that zymograms allowed some species to be readily recognized despite morphological variation. This was also seen in the dendrograms where, in addition, groupings based on ecological or sexual criteria could be distinguished.
Summary: Variants capable of growth at pH 7·0 were obtained from 174 alkalophilic strains (optimum pH 9·7) of the genus Bacillus by successive transfer on media with decreasing pH values. Nearly half of the pH 7·0 variants were like strains of the B. firmus–B. lentus complex. The remaining pH 7·0 variants grew at 50 °C and were assigned to two groups closely related to B. lentus.