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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 5

Isolation and characterization of a novel species and reclassification of Hitch . 2022 as comb. nov. No Access

Abstract

Obligately anaerobic, Gram-stain-negative, wavy rods, strains 17YCFAHCo10, 18YCFAH0.3Co2 and 19YCFAH0.3Co2, were isolated from faecal samples of healthy Japanese people. The three isolates showed the highest 16S rRNA gene sequence similarity to WCA3-601-WT-6H (99.2–100 %) and f_CXY (99.2–99.7 %). The 16S rRNA gene sequence analysis showed that the three isolates formed a cluster with WCA3-601-WT-6H. Strain 19YCFAH0.3Co2 formed a subcluster with the type strain of and did not form a cluster with the other two isolates. f_CXY also formed a cluster with WCA3-601-WT-6H and three isolates. The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain 19YCFAH0.3Co2 and WCA3-601-WT-6H were higher (72 % dDDH and 97 % ANI) than the cut-off values for species delimitation, indicating that strain 19YCFAH0.3Co2 is . On the other hand, the dDDH and ANI values between strains 17YCFAHCo10 and 18YCFAH0.3Co2 and the type strain of were lower (<34 % dDDH and <87 % ANI) than the cut-off values for species delimitation, indicating that these two isolates are different species from . The percentage of conserved proteins and the average amino acid identity values support the assignment of the isolates to the genus . Strains 17YCFAHCo10 and 18YCFAH0.3Co2 could be distinguished from by their inability to ferment melibiose and ribose and lack of activity for -glucuronidase. In addition, the dDDH and ANI values between two strains (17YCFAHCo10 and 18YCFAH0.3Co2) and f_CXY were higher (>78 % dDDH and >97 % ANI), indicating these two strains and are the same species. As the genus has priority, is transferred to the genus as comb. nov. The type strain of is f_CXY (=JCM 34988=DSM 107528).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Brotolimicola , elongation , human faeces , reclassification and Waltera
Funding
This study was supported by the:
  • the Committee on Classification and Culturing for Lactic Acid Bacteria and Gut Bacteria in Japan Society for Lactic Acid Bacteria, which is established with the financial support by the Institute for Fermentation, Osaka（IFO）: GK-2022-001
    • Principle Award Recipient: AtsushiHisatomi
  • Japan Agency for Medical Research and Development (Award JP23ae0121035)
    • Principle Award Recipient: MitsuoSakamoto
  • Japan Agency for Medical Research and Development (Award JP19gm6010007)
    • Principle Award Recipient: MitsuoSakamoto
© 2024 The Authors
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Isolation and characterization of a novel Waltera species and reclassification of Brotolimicola acetigignens Hitch et al. 2022 as Waltera acetigignens comb. nov.
Int J Syst Evol Microbiol 74, 006381 (2024); https://doi.org/10.1099/ijsem.0.006381
/content/journal/ijsem/10.1099/ijsem.0.006381
/content/journal/ijsem/10.1099/ijsem.0.006381
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