1887

Abstract

Summary

Sixty-two isolates of from canned foods were examined for cultural properties, heat resistance and DNA-DNA homology to type 190. Sporulation was observed in most of 21 umbonate and rhizoidal colony-forming strains (colony-type I strains), but not in most of the 41 strains with convex and circular or crenate colonies with a mat to semi-glossy surface (colony-type II strains). More than half of the latter strains showed much higher heat resistance than the rhizoidal colony-forming strains. The DNA isolated from colony-type II strains was 81% or more homologous to 190 DNA, forming duplexes which had thermostabilities similar to homologous duplexes of strain 190 DNA. Colony-type I strains differed from by 30 to 40% DNA homology and the DNA duplexes formed between these strains and strain 190 showed Δ values of 7·0 C when compared with the of homologous DNA duplexes of strain 190.

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/content/journal/micro/10.1099/00221287-100-2-395
1977-06-01
2024-04-18
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References

  1. Anema P. J., Geers J. M. 1973; Controlling the sporulation of Clostridium sporogenes and the heat resistance of the spores. Journal of Applied Bacteriology 36:553–558
    [Google Scholar]
  2. Aschehoung V., Jansen E. 1950; Studies on putrefactive anaerobes as spoilage agents in canned foods. Food Research 15:62–67
    [Google Scholar]
  3. Bulloch W., Bullock W. E., Douglas S. R., Henry K., Mcintosh J., O’Brien R. A. 1919; Report on the anaerobic infections of wounds and the bacteriological and serological problems arising therefrom. Medical Research Council Special Series 39:39–40
    [Google Scholar]
  4. Cummins C. S., Johnson J. L. 1971; Taxonomy of the clostridia: wall composition and DNA homologies in Clostridium butyricum and other butyric acid-producing clostridia. Journal of Microbiology 67:33–46
    [Google Scholar]
  5. Holdeman L. V., Moore W. E. C. editors 1975 Anaerobe Laboratory Manual, 3rd edn.. Blacksburg, Virginia: Virginia Polytechnic Institute and State University;
    [Google Scholar]
  6. Iida H. 1964; Experimental studies on toxin production of Clostridium botulinum. I. Japanese Journal of Bacteriology 11:458–461 in Japanese
    [Google Scholar]
  7. Kato H., Nakamura S., Serikawa T., Matsuda N. 1975; Clostridium sporogenes strains from canned foods. Japanese Journal of Bacteriology 30:67 in Japanese
    [Google Scholar]
  8. Kiritani K., Mitsui N., Nakamura S., Nishida S. 1973; Numerical taxonomy of Clostridium botulinum and Clostridium sporogenes strains and their susceptibilities to induced lysins and mitomycin C. Japanese Journal of Microbiology 17:361–372
    [Google Scholar]
  9. Lee W. H., Riemann H. 1970; The genetic relatedness of proteolytic Clostridium botulinum strains. Journal of General Microbiology 64:85–90
    [Google Scholar]
  10. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  11. Meyer K. F., Lang O. W. 1926; A highly heat-resistant sporulating anaerobic bacterium Clostridium caloritolerans, n.sp. Journal of Infectious Diseases 39:321–327
    [Google Scholar]
  12. Nakamura S., Nishida S. 1974; Reinvestigations of the relationship between sporulation, heat resistance and some biochemical properties in strains of Clostridium perfringens. Journal of Medical Microbiology 7:451–457
    [Google Scholar]
  13. Nakamura S., Shimamura T., Hayashi H., Nishida S. 1974; Reinvestigation of the taxonomy of C.bifermentans and C.sordellii. Journal of Medical Microbiology 8:299–309
    [Google Scholar]
  14. Oakley C. L. 1962; Soluble bacterial antigens as discriminants in classification. In Microbial Classification pp. 242–248 Ainsworth G. C., Sneath P. H. A. Edited by Cambridge University Press:
    [Google Scholar]
  15. PrÉvot A. R., Turpin A., Kaiser P. 1967 Les Bactéries Anaerobies pp. 1096–1097 Paris: Dunod;
    [Google Scholar]
  16. Smith L. DS. 1975 The Pathogenic Anaerobic Bacteria, 2nd edn.. p. 314 Springfield: Charles C. Thomas, Publisher;
    [Google Scholar]
  17. Takumi K., Kawata T. 1970; Chemical composition of the cell walls of Clostridium botulinum type A. Japanese Journal of Microbiology 14:57–63
    [Google Scholar]
  18. Wirtz K. 1908; Eine einfache Art der Sporenfärbung. Zentralblatt für Bacteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originale) A46:727–728
    [Google Scholar]
  19. Wu J. I. J., Riemann H., Lee W. H. 1972; Thermal stability of the deoxyribonucleic acid hybrids between the proteolytic strains of Clostridium botulinum and Clostridium sporogenes. Canadian Journal of Microbiology 18:97–99
    [Google Scholar]
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