1887

Abstract

The properties of a temperature-sensitive mutant (ts39) of 8235 are described. After transfer to the restrictive temperature (42 °C), absorbance increased 10-to 20-fold but DNA content did not increase beyond 150 to 200% and cell division continued at a greatly reduced rate. On transfer back to the permissive temperature, both cell division and DNA synthesis resumed if the transfer occurred after less than 120 min at 42 °C. Resumption of DNA replication was blocked by chloramphenicol (100 ?g ml). The results are discussed with reference to possible defects in DNA replication.

Replication of the plasmids pI and pT and the chromosome were affected to a similar extent in ts39. Growth at 42 °C resulted in the appearance of an increased amount of pI DNA in a form that sedimented slowly in a sucrose gradient.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-106-2-361
1978-06-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/106/2/mic-106-2-361.html?itemId=/content/journal/micro/10.1099/00221287-106-2-361&mimeType=html&fmt=ahah

References

  1. Arai T., Clowes R. 1974; Replication of stringent and relaxed plasmids. In Microbiology-1974 pp 141–155 Editor Schlessinger D., Washington D.C. American Society for Microbiology.;
    [Google Scholar]
  2. Beyersmann D., Schlicht M., Schuster H. 1971; Temperature-sensitive initiation of DNA replication in a mutant of Escherichia coli k12. Molecular and General Genetics 111:145–158
    [Google Scholar]
  3. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of DNA. Biochemical Journal 62:315–323
    [Google Scholar]
  4. Chopra I., Bennett P. M., Lacey R. W. 1973; A variety of staphylococcal plasmids present as multiple copies. Journal of General Microbiology 79:343–345
    [Google Scholar]
  5. Cooper S., Helmstetter C. E. 1968; Chromosome replication and the division cycle of Escherichia coli B/r. Journal of Molecular Biology 31519–540
    [Google Scholar]
  6. Evans I. M., Eberle H. 1975; Accumulation of the capacity for initiation of DNA replication in Escherichia coli. Journal of Bacteriology 121:883–891
    [Google Scholar]
  7. Fangman W. L., Novick A. 1968; Characterization of two bacterial mutants with temperature- sensitive synthesis of DNA. Genetics 60:1–17
    [Google Scholar]
  8. Hirota Y., Ryter A., Jacob F. 1968; Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division. Cold Spring Harbor Symposia on Quantitative Biology 33:677–693
    [Google Scholar]
  9. Katchatourians G. C., Clark D. J. 1970; Regulation of cell division and DNA replication in Escherichia coli. Bacteriological Proceedings 71:
    [Google Scholar]
  10. Kohiyama M. 1968; DNA synthesis in temperature-sensitive mutants of Escherichia coli. Cold Spring Harbor Symposia on Quantitative Biology 33:317–324
    [Google Scholar]
  11. Lacey R. W. 1975; Antibiotic resistance plasmids of Staphylococcus aureus and their clinical importance. Bacteriological Reviews 39:1–32
    [Google Scholar]
  12. Lark K. G. 1966; Regulation of chromosome replication and segregation in bacteria. Bacteriological Reviews 30:3–32
    [Google Scholar]
  13. Lark K. G. 1973; Initiation and termination of bacterial deoxyribonucleic acid replication in low concentrations of chloramphenicol. Journal of Bacteriology 113:1066–1069
    [Google Scholar]
  14. Maaloe O., Hanawalt P. C. 1961; Thymine deficiency and the normal DNA replication cycle. I. Journal of Molecular Biology 3:144–155
    [Google Scholar]
  15. Marunouchi T., Messer W. 1973; Replication of a specific terminal chromosome segment in Escherichia coli which is required for cell division. Journal of Molecular Biology 78:211–228
    [Google Scholar]
  16. Measures J. C. 1973; The relationships between the protoplast membrane and deoxyribonucleic acid in Staphylococcus aureus. D.Phil. thesis University of Oxford.;
    [Google Scholar]
  17. Mitsuhashi A., Hashimoto H., Kono M., Murimura M. 1965; Drug resistance in staphylococci. II. Joint elimination and joint transduction of the determinants of penicillinase production and resistance to macrolide antibiotics. Journal of Bacteriology 89:988–992
    [Google Scholar]
  18. Novick R. P. 1963; Analysis by transduction of mutations affecting penicillinase formation in Staphylococcus aureus. Journal of General Microbiology 33:121–136
    [Google Scholar]
  19. Novick R. P. 1974; Studies on plasmid replication. III. Isolation and characterization of replication-defective mutants. Molecular and General Genetics 135:131–147
    [Google Scholar]
  20. Novick R. P., Brodsky R. 1972; Studies on plasmid replication. I. Plasmid incompatibility and establishment in S. aureus. Journal of Molecular Biology 68:285–302
    [Google Scholar]
  21. Novick R. P., Wyman L., Bouanchaud D., Murphy E. 1975; Plasmid life cycles in Staphylococcus aureus. In Microbiology- 1974 pp. 115–129 Editor Schlessinger D., Washington D.C. American Society for Microbiology.;
    [Google Scholar]
  22. Ruby C., Novick R. P. 1975; Plasmid interactions in Staphylococcus aureus: nonadditivity of compatible plasmid DNA pools. Proceedings of the National Academy of Sciences of the United States of America 72:5031–5035
    [Google Scholar]
  23. Rush M., NOVICK R. P., DELAP R. 1975; Detection and quantitation of Staphylococcus aureus penicillinase plasmid DNA by reassociation kinetics. Journal of Bacteriology, 124:1417–1423
    [Google Scholar]
  24. SHEEHY R. J., NOVICK R. P. 1975; Studies on plasmid replication. V. Replicative intermediates. Journal of Molecular Biology 93:237–253
    [Google Scholar]
  25. SUEOKA N., YOSHIKAWA H. 1965; The chromosome of Bacillus subtilis. I. The theory of marker frequency analysis. Genetics 52:747–757
    [Google Scholar]
  26. THOMAS C. M., Dyke K. G. H. 1978; Isolation and transduction analysis of temperature-sensitive mutants of Staphylococcus aureus defective in DNA replication. Journal of General Microbiology 106:41–47
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-106-2-361
Loading
/content/journal/micro/10.1099/00221287-106-2-361
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error