The Fluorescent Pigment of Pseudomonas fluorescens: Biosynthesis, Purification and Physicochemical Properties MEYER, J. M. and ABDALLAH, M. A.,, 107, 319-328 (1978), doi = https://doi.org/10.1099/00221287-107-2-319, publicationName = Microbiology Society, issn = 1350-0872, abstract= The biosynthesis of a yellow–green, fluorescent, water-soluble pigment by Pseudomonas fluorescens occurred only when the bacteria were iron-deficient and was not directly influenced by the nature of the organic carbon source. The pigment formed a very stable Fe3+ complex and was purified in this form. Pseudomonas fluorescens produced only one molecular species of fluorescent pigment; however, its lability under mild alkaline conditions led to the formation of several pigmented decomposition products. The spectral properties of the pure pigment, its molecular weight (1500 ± 75) and its stability constant for Fe3+ (of the order of 1032) were determined. Both its biosynthesis and its chemical properties (formation of a stable Fe3+ complex) suggest that the fluorescent pigment is a desferrisiderophore., language=, type=