1887

Abstract

Synchronous cultures of B were produced under various environmental conditions. Analysis of the cell number data permitted the characterization of the generation time distribution for these organisms and the estimation of the mother-daughter generation time correlation coefficients. For all growth conditions, the distribution of generation times was found to be symmetrical with a coefficient of variation of 0·22±0·02. The mother-daughter generation time correlation coefficient was significantly negative at doubling times between 40 and 64 min. However, the results for a culture growing in succinate medium at 37°C, which had a significantly greater generation time, yielded a correlation coefficient close to zero. Within the range of temperatures studied (26 to 37°C), no significant effect on the correlation coefficient was observed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-115-1-69
1979-11-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/115/1/mic-115-1-69.html?itemId=/content/journal/micro/10.1099/00221287-115-1-69&mimeType=html&fmt=ahah

References

  1. Britten R.J., Roberts R.B. 1960; High resolution density gradient sedimentation analysis.. Science 131:32–33
    [Google Scholar]
  2. Burdett I.D.J., Murray R.G.E. 1974; Electron microscope study of septum formation in Escherichia coli strains B and B/r during synchronous growth.. Journal of Bacteriology 119:1039–1056
    [Google Scholar]
  3. Gudas L.J., Pardee A.B. 1974; Deoxyribonucleic acid synthesis during the division cycle of Escherichia coli: a comparison of strains B/r, K–12, 15, and 15T under conditions of slow growth.. Journal of Bacteriology 117:1216–1223
    [Google Scholar]
  4. Harvey J.D. 1972a; Synchronous growth of cells and the generation time distribution.. Journal of General Microbiology 70:99–108
    [Google Scholar]
  5. Harvey J.D. 1972b; Parameters of the generation time distribution of Escherichia coli B/r.. Journal of General Microbiology 70:109–114
    [Google Scholar]
  6. Helmstetter C.E. 1969; Methods for studying the microbial division cycle.. Methods in Microbiology 1:327–363
    [Google Scholar]
  7. Helmstetter C.E., Cummings D.F. 1964; An improved method for the selection of bacterial cells at division.. Biochimica et biophysica acta 82:608–610
    [Google Scholar]
  8. Kelly C.D., Rahn O. 1932; The growth rate of individual bacterial cells.. Journal of Bacteriology 23:147–153
    [Google Scholar]
  9. Kubitschek H.E. 1962; Normal distribution of cell generation rate.. Experimental Cell Research 26:439–450
    [Google Scholar]
  10. Kubitschek H.E. 1966; Generation times: ancestral dependence and dependence upon cell size.. Experimental Cell Research 43:30–38
    [Google Scholar]
  11. Kubitschek H.E. 1971; The distribution of cell generation times.. Cell Tissue Kinetics 4:113–122
    [Google Scholar]
  12. Mitchison J.M., Vincent W.S. 1965; Preparation of synchronous cultures by sedimentation.. Nature; London: 205987–989
    [Google Scholar]
  13. Plank L.D. 1978 Generation time statistics of Escherichia coli using synchronous culture techniques. D.Phil. thesis University of Waikato, New Zealand:
    [Google Scholar]
  14. Powell E.O. 1955; Some features of the generation times of individual bacteria.. Biometrika 42:16–44
    [Google Scholar]
  15. Powell E.O. 1956; Growth rate and generation time of bacteria, with special reference to continuous culture.. Journal of General Microbiology 15:492–511
    [Google Scholar]
  16. Powell E.O. 1958; An outline of the pattern of bacterial generation times.. Journal of General Microbiology 18:382–417
    [Google Scholar]
  17. Powell E.O., Errington F.P. 1963; Generation times of individual bacteria: some corroborative measurements.. Journal of General Microbiology 31:315–327
    [Google Scholar]
  18. Princen L.H., Kwolek W.F. 1966; Coincidence corrections for particle size determinations with the Coulter counter.. Review of Scientific Instruments 36:646–653
    [Google Scholar]
  19. Rackham S.J. 1977 A pulse height analyser for displaying Coulter counter particle size distributions. M.Sc. thesis University of Waikato, New Zealand.:
    [Google Scholar]
  20. Schaechter M., Williamson J.P., Hood J.R. Jr Koch A. L. 1962; Growth, cell and nuclear divisions in some bacteria.. Journal of General Microbiology 29:421–434
    [Google Scholar]
  21. Shehata T.E., Marr A.G. 1970; Synchronous growth of enteric bacteria.. Journal of Bacteriology 103:789–792
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-115-1-69
Loading
/content/journal/micro/10.1099/00221287-115-1-69
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error