1887

Abstract

Cerulenin inhibited the secretion of extracellular proteins by washed cell suspensions of α-Amylase and protease secretion were inhibited by 80% and 75%, respectively, over 3 h. Cerulenin at 100 g ml inhibited incorporation of [1,2-C]- acetate into intracellular lipid by about 75% without affecting cell growth, total protein synthesis or membrane protein synthesis. The inhibitory effect of cerulenin on α-amylase and protease secretion could be partially reversed if cell suspensions were supplemented with either fatty acids prepared from the lipids extracted from or various individual pure fatty acids. Cerulenin significantly altered the ratio of lipid to protein in isolated membranes. However, this alteration was not affected by adding fatty acids which restored enzyme secretion. These results suggest that cerulenin may affect the availability of lipid directly concerned with the secretion process. The differential effect of cerulenin on the production of extracellular proteins and membrane proteins also suggests that the synthesis of these two classes of proteins occurs via mechanisms that differ.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-118-1-179
1980-05-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/118/1/mic-118-1-179.html?itemId=/content/journal/micro/10.1099/00221287-118-1-179&mimeType=html&fmt=ahah

References

  1. Altenbern R. A. 1977; Extreme sensitivity of staphylococcal enterotoxin B and C production to inhibition by cerulenin. Antimicrobial Agents and Chemotherapy 11:906–908
    [Google Scholar]
  2. Blobel G., Dobberstein B. 1975; Transfer of proteins across membranes. Journal of Cell Biology 67:835–851
    [Google Scholar]
  3. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  4. Both G. W., Mcinnes J. L., Hanlon J. E., May B. K., Elliott W. H. 1972; Evidence for an accumulation of messenger RNA specific for extracellular protease and its relevance to the mechanism of enzyme secretion in bacteria. Journal of Molecular Biology 67:199–217
    [Google Scholar]
  5. Caulfield M., Chopra I., Melling J., Berkeley R. C. W. 1976; The effect of cerulenin on the liberation of extracellular inducible levansucrase by Bacillus subtilis . Proceedings of the Society for General Microbiology 3:91–92
    [Google Scholar]
  6. Fishman Y., Rottem S., Citri N. 1978; Evidence linking penicillinase formation and secretion to lipid metabolism in Bacillus licheniformis . Journal of Bacteriology 134:434–439
    [Google Scholar]
  7. Goldfine H., Harley J. B., Wyke J. A. 1978; Effects of inhibitors of lipid synthesis on the replication of Rous sarcoma virus. A specific effect of cerulenin on the processing of major non-glycosylated viral structural proteins. Biochimica et biophysica acta 512:229–240
    [Google Scholar]
  8. Konings W. N., Bisschop A., Veenhuis M., Vermeulen C. A. 1973; New procedure for the isolation of membrane vesicles of Bacillus subtilis and an electron microscopy study of their ultrastructure. Journal of Bacteriology 116:1456–1465
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  10. Lazdunski A., Murgier M., Lazdunski C. 1979; Phospholipid synthesis-dependent activity of aminopeptidase N in intact cells of Escherichia coli . Journal of Molecular Biology 128:127–141
    [Google Scholar]
  11. O’Connor R., Elliott W. H., May B. K. 1978; Modulation of an apparent mRNA pool for extracellular protease in Bacillus amyloliquefaciens . Journal of Bacteriology 136:24–34
    [Google Scholar]
  12. Omura S. 1976; The antibiotic cerulenin, a novel tool for biochemistry as an inhibitor of fatty acid synthesis. Bacteriological Reviews 40:681–697
    [Google Scholar]
  13. Paton J. C., May B. K., Elliott W. H. 1978a; Membrane phospholipid asymmetry in Bacillus amyloliquefaciens . Journal of Bacteriology 135:393–401
    [Google Scholar]
  14. Paton J. C., Mcmurchie E. J., May B. K., Elliott W. H. 1978b; Effect of growth temperature on membrane fatty acid composition and susceptibility to cold shock of Bacillus amyloliquefaciens . Journal of Bacteriology 135:754–759
    [Google Scholar]
  15. Rinderknecht J., Geokas M. C., Silverman P., Kaverback B. J. 1968; A new ultrasensitive method for the determination of proteolytic activity. Clinica chimica acta 21:197–203
    [Google Scholar]
  16. Rothman J. E., Lenard J. 1977; Membrane asymmetry. Science 195:743–753
    [Google Scholar]
  17. Sanders R. L., May B. K. 1975; Evidence for the extrusion of unfolded extracellular enzyme polypeptide chains through membranes of Bacillus amyloliquefaciens . Journal of Bacteriology 123:806–814
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-118-1-179
Loading
/content/journal/micro/10.1099/00221287-118-1-179
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error