1887

Abstract

Ansamitocins inhibited cell division in W at low concentrations and caused cells to become more rounded. Exposure to ansamitocins for 5 h or more resulted in a burst of synchronous division at 100 min after removal of the antibiotics with a maximum division index of 70 %. A second burst of synchronous division occurred 300 min after removal with a peak division index of 55 %. The rounding of the cell shape was restored by the completion of the first division. When RNA or protein synthesis was blocked by chromomycin A or cycloheximide, the first division was suppressed. When DNA synthesis was blocked by methotrexate plus uridine, synchronous division still occurred; furthermore, the DNA content of cells did not increase before the first division. These findings suggest that the first synchronous division requires RNA and protein synthesis but does not require DNA synthesis. Interference by ansamitocins with the function of microtubule systems in is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-118-2-411
1980-06-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/118/2/mic-118-2-411.html?itemId=/content/journal/micro/10.1099/00221287-118-2-411&mimeType=html&fmt=ahah

References

  1. Anderson H. A., Rasmussen L., Zeuthen E. 1975; Cell division and DNA replication in synchronous Tetrahymena cultures.. Current Topics in Microbiology and Immunology 72:1–20
    [Google Scholar]
  2. Blumenthal L. K., Zahler S. A. 1962; Index for measurement of synchronization of cell population.. science 135:724
    [Google Scholar]
  3. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.. Biochemical Journal 62:315–323
    [Google Scholar]
  4. Burton K. 1968; Determination of DNA concen-tration with diphenylamine.. Methods in Enzymology 12B:163–166
    [Google Scholar]
  5. Everhart L. P. Jr 1972; Methods with Tetra-hymena.. Methods in Cell Physiology 5:219–288
    [Google Scholar]
  6. Higashide E., Asai M., Ootsu K., Tanida S., Kozai Y., Hasegawa T., Kishi T., Sugino Y., Yoneda M. 1977; Ansamitocins, a novel group of maytansinoid antibiotics with antitumor properties.. Nature; London: 270721–722
    [Google Scholar]
  7. Kennedy J. R., Zimmerman A. M. 1970; The effects of high hydrostatic pressure on the micro-tubules of Tetrahymena pyriformis.. Journal of Cell Biology 47:568–576
    [Google Scholar]
  8. Kupchan S. M., Komoda Y., Court W. A., Thomas J. G., Smith R. M., Karin A., Gilmore C. J., Haltiwanger R. C., Bryan R. F. 1972; Maytansine, a novel antileukemic ansa macrolide from Maytenus ovatus.. Journal of the American Chemical Society 94:1354–1356
    [Google Scholar]
  9. Lown J. W., Majumdar K. C., Meyers A. I., Hecht A. 1977; Studies related to antitumor antibiotics. X. Reactions of maytansine and its analogs with DNA in vitro.. Bioorganic Chemistry 6:453–463
    [Google Scholar]
  10. Rannestad J. 1974; The regeneration of cilia in partially deciliated Tetrahymena.. Journal of Cell Biology 63:1009–1017
    [Google Scholar]
  11. Rosenbaum J. L., Carlson K. 1968; Cilia regeneration in Tetrahymena and its inhibition by colchicine.. Journal of Cell Biology 40:415–425
    [Google Scholar]
  12. Rosenbaum J. L., Moulder J. F., Ringo B. L. 1969; Flagella elongation and shortening in Chlamydomonas. The use of cycloheximide and colchicine to study the synthesis and assembly of flagella proteins.. Journal of Cell Biology 41:600–619
    [Google Scholar]
  13. Sedgley N. N., Stone G. E. 1969; DNA synthesis in vinblastine synchronized Tetrahymena.. Experimental Cell Research 56:174–177
    [Google Scholar]
  14. Stephens R. E., Edds K. R. 1976; Microtubules: structure, chemistry and function.. Physiological Reviews 56:709–777
    [Google Scholar]
  15. Stone G. E. 1968a; Method for reversible inhibition of cell division in Tetrahymena pyriformis using vinblastine sulfate.. Methods in Cell Physiology 3:161–170
    [Google Scholar]
  16. Stone G. E. 1968b; Synchronized cell division in Tetrahymena pyriformis following inhibition with vinblastine.. Journal of Cell Biology 39:556–563
    [Google Scholar]
  17. Tanida S., Higashide E., Yoneda Y. 1979; Inhibition of cilia regeneration of Tetrahymena by ansamitocins, new antitumor antibiotics.. Antimicrobial Agents and Chemotherapy 16:101–103
    [Google Scholar]
  18. Villadsen I. S., Zeuthen E. 1970; Synchronization of DNA synthesis in Tetrahymena populations by temporary limitation of access to thymine compounds.. Experimental Cell Research 61:302–310
    [Google Scholar]
  19. Wolpert-Defillipes M. K., Adamson R. H., Cysyk R. L., Johns D. G. 1975; Initial studies on the cytotoxic action of maytansine, a novel ansa macrolide.. Biochemical Pharmacology 24:751–754
    [Google Scholar]
  20. Wunderlich F., Peyk D. 1969; Antimitotic agents and macronuclear division of ciliates.. Experimental Cell Research 57:142–144
    [Google Scholar]
  21. Zeuthen E. 1968; Thymine starvation by inhibition of uptake and synthesis of thymine compounds in Tetrahymena.. Experimental Cell Research 50:37–46
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-118-2-411
Loading
/content/journal/micro/10.1099/00221287-118-2-411
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error