1887

Abstract

A transposon, Tn, encoding the gentamicin acetyltransferase AAC(3) was found on two gentamicin R plasmids of IncW at Hammersmith Hospital. Transposon Tn has a molecular mass of 5·8 megadaltons and gives a characteristic 2·4 megadalton fragment on digestion with RI. The appearance of gentamicin resistance on a transposon will increase the chances of spread of this gene.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-121-1-259
1980-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/121/1/mic-121-1-259.html?itemId=/content/journal/micro/10.1099/00221287-121-1-259&mimeType=html&fmt=ahah

References

  1. Bachmann B.J. 1972; Pedigrees of some mutant strains of Escherichia coli K12.. Bacteriological Reviews 36:525–557
    [Google Scholar]
  2. Blohm D., Goebel W. 1978; Restriction map of the antibiotic resistance plasmid Rldrd-19 and its derivatives pKN102 (R1drd-1932) and R1drd-16 for the enzymes Bam RI, Hind III, Eco RI and Sal I.. Molecular and General Genetics 167:119–127
    [Google Scholar]
  3. Datta N., Hedges R.W. 1972; Trimethoprim resistance conferred by W plasmids in Enterobacteriaceae.. Journal of General Microbiology 72:349–356
    [Google Scholar]
  4. Datta N., Hughes V.M., Nugent M.E., Richards H. 1979; Plasmids and transposons, their stability and mutability in bacteria isolated during an outbreak of hospital infection.. Plasmid 2:182–196
    [Google Scholar]
  5. Datta N., Dacey S., Hughes V., Knight S., Richards H., Williams G., Casewell M., Shannon K.P. 1980; Distribution of genes for trimethoprim and gentamicin resistance in bacteria and their plasmids in a general hospital.. Journal of General Microbiology 118:495–508
    [Google Scholar]
  6. Eckhardt T. 1978; A rapid method for the identification of plasmid DNA in bacteria.. Plasmid 1:584–588
    [Google Scholar]
  7. Jacob A.E., Shapiro J.A., Yamamoto L., Smith D.I., Cohen S.N., Berg D. 1977; Plasmids studied in Escherichia coli and other enteric bacteria.. In DNA Insertion Elements, Plasmids and Episomes pp. 607–638 Bukhari A.I., Shapiro J.A., Adhya S.L. Edited by New York:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  8. Nugent M.E., Bone D.H., Datta N. 1979; A transposon, Tn732, encoding gentamicin/ tobramycin resistance.. Nature; London: 282422–423
    [Google Scholar]
  9. Rubens C.E., Mcneill W.F., Farrar W.E. 1979a; Transposable plasmid deoxyribonucleic acid sequence in Pseudomonas aeruginosa which mediates resistance to gentamicin and four other antimicrobial agents.. Journal of Bacteriology 139:877–882
    [Google Scholar]
  10. Rubens C.E., Mcneill W.F., Farrar W.E. 1979b; Evolution of multiple antibiotic resistance plasmids mediated by transposable plasmid DNA sequences.. Journal of Bacteriology 140:713–719
    [Google Scholar]
  11. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis.. Journal of Molecular Biology 98:503–517
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-121-1-259
Loading
/content/journal/micro/10.1099/00221287-121-1-259
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error