f Sporulation of Aspergillus niger and Aspergillus ochraceus in Continuous Submerged Liquid Culture
- Authors: A. J. BRODERICK, R. N. GREENSHIELDS
- Microbiology, September 1981 126: 193-202, doi: 10.1099/00221287-126-1-193
- Subject: Physiology And Growth
- Published Online:
Sporulation of Aspergillus niger and Aspergillus ochraceus was induced in a continuous tower fermenter by restricting growth by nutrient limitation. Shock carbon limitation produced no sporulation, but the gradual decrease of sucrose or starch supply to A. niger produced slight sporulation. Gradual nitrate limitation produced no sporulation, while a shock decrease in nitrate concentration caused heavy sporulation of both organisms. The previously unobserved morphology of the sporulating structures produced was much simplified under nitrate limitation, but similar to sub-aerial morphology under carbon limitation. Maintenance energy values for sucrose and starch were calculated for A. niger and for starch for A. ochraceus. The continuous tower fermenter system was found to be ideal for controlling organism morphology and thus sporulation.
© Society for General Microbiology 1981 | Published by the Microbiology Society
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