Transduction of Escherichia coli trp Genes in Salmonella typhimurium and Effect of N-Methyl-N′-Nitro-N-Nitrosoguanidine on Transduction with Heterogenotic DNA
Mergeay, Max and Gerits, Jozef,, 129, 321-335 (1983),
doi = https://doi.org/10.1099/00221287-129-2-321,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= SUMMARY: P1Kc-mediated transduction of Escherichia coli trp genes occurred at a frequency of about 10−8 in Salmonella typhimurium trp strains carrying mutations determining sensitivity to P1 and a low level of restriction enzymes. Heterospecific transductants were analysed by using them as donors in second-stage transductions mediated by bacteriophage KB int. One class of heterospecific transductants had the phenotype Trp+ Pro− but were extremely unstable and reverted at high frequency (up to 80%) to the parental phenotype. The Trp+ Pro− phenotype probably represents insertions of the E. coli trp genes in the S. typhimurium pro genes. It was stable in a RecA background.,
language=,
type=