1887

Abstract

Lipopolysaccharides extracted by phenol-water from nine strains of Gram-positive anaerobic bacteria ( and ), have been examined by means of pyrolysis gas chromatography. Lipopolysaccharides were fragmented into a group of low molecular weight components and four characteristic high molecular fractions probably consisting of hydrocarbons from the lipid part of the material. The latter fractions were specific for each of the genera tested. At the species level characteristic differences were also found although a limited number of strains were tested. Due to the high reproducibility of the technique, the potential of using the method in differentiating Gram-negative anaerobic bacteria was indicated.

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1983-03-01
2024-04-19
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References

  1. Buchanan R. E., Gibbons N. E. 1974 Bergey’s Manual of Determinative Bacteriology, 8th edn. Baltimore: Williams and Wilkins;
    [Google Scholar]
  2. Dahlén G., Bergenholtz G. 1980; Endotoxin activity in teeth with necrotic pulps. Journal of Dental Research 59:1033–1040
    [Google Scholar]
  3. Drucker D. B. 1981 Microbiological Applications of Gas Chromatography pp. 364–399 Cambridge: Cambridge University Press;
    [Google Scholar]
  4. Emswiler B. S., Kotula A. W. 1978; Differentiation of Salmonella serotypes by pyrolysis gas liquid chromatography of cell fragments. Applied and Environmental Microbiology 35:97–104
    [Google Scholar]
  5. Ericsson I. 1975 Qualitative, and kinetic studies of salts and polymers with a new pyrolyser in combination with a gas chromatograph. Thesis, University of Lund, Sweden
    [Google Scholar]
  6. Ericsson I. 1977; Pyrolysis gas chromatography - a potential technique for characterization of complex organic material. Acta pathologica microbiologica scandinavica B259:37–42
    [Google Scholar]
  7. Ericsson I., Larsson L., Mårdh P.-A. 1977; Pyrolysis gas chromatography of microorganisms. Influence of various pyrolysis parameters on the yield of volatile organic products. Acta pathologica microbiologica scandinavica B259:43–47
    [Google Scholar]
  8. Fabricius L., Dahlén G., Holm S. E., Möller Å.J.R. 1982; Influence of combinations of oral bacteria on periapical tissues of monkeys. Scandinavian Journal of Dental Research 90:200–206
    [Google Scholar]
  9. Finegold S. M. 1977 Anaerobic Bacteria in Human Disease. New York: Academic Press;
    [Google Scholar]
  10. Fredriksen G., Hofstad T. 1978; Chemotypes of Fusobacterium nucleatum lipopolysaccharides. Acta pathologica microbiologica scandinavica B86:41–45
    [Google Scholar]
  11. Fritsche C. D. 1974; Zur Struktur der Lipoide des Genus Bacteroides. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originate A) 228:100–106
    [Google Scholar]
  12. Gutteridge C. S., Norris J. R. 1979; The application of pyrolysis techniques to the identification of micro-organisms. Journal of Applied Bacteriology 47:5–43
    [Google Scholar]
  13. Haddadin J. M., Stirland R. M., Preston N. W., Collard P. 1973; Identification of Vibrio cholerae by pyrolysis gas liquid chromatography. Applied Microbiology 25:40–43
    [Google Scholar]
  14. Hase I., Hofstad T., Rietschel E. T. 1977; Chemical structure of the lipid A component of lipopolysaccharides from Fusobacterium nucleatum. Journal of Bacteriology 129:9–14
    [Google Scholar]
  15. Hofstad T. 1978; Chemotypes of Veillonella lipopolysaccharides. Acta pathologica microbiologica scandinavica B86:47–50
    [Google Scholar]
  16. Hofstad T., Kristoffersen T. 1970; Chemical characteristics of endotoxin from Bacteroides fragilis NCTC 9343. Journal of General Microbiology 61:15–19
    [Google Scholar]
  17. Hofstad T., Skaug N. 1980; Fatty acid and neutral sugars present in lipopolysaccharides isolated from Fusobacterium species. Acta pathologica microbiologica scandinavica B88:115–120
    [Google Scholar]
  18. Holdeman L. V., Moore W. E. C. 1975 Anaerobe Laboratory Manual, 3rd edn. V.P.I. Anaerobe Laboratory, Blacksburg, Virginia, U.S.A.: Virginia Polytechnic Institute and State University;
    [Google Scholar]
  19. Jantzen E., Hofstad T. 1981; Fatty acids of Fusobacterium species: taxonomy implications. Journal of General Microbiology 123:163–171
    [Google Scholar]
  20. Kauffmann F. 1961 Die Bakteriologie der Salmonella Species. Copenhagen: Munksgaard;
    [Google Scholar]
  21. Linder L., Holme T., Frostell G. 1974; Hyaluronidase and aminopeptidase activity in cultures of Streptococcus mitis ATCC 903. Acta pathologica microbiologica scandinavica B82:521–526
    [Google Scholar]
  22. Lüderitz O., Staub A. M. 1966; Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bacteriological Reviews 30:192–244
    [Google Scholar]
  23. Miyagawa E., Suto T. 1980; Cellular fatty acid composition in Bacteroidesoralis and Bacteroidesruminicola. Journal of General and Applied Microbiology 26:331–343
    [Google Scholar]
  24. Miyagawa E., Azuma R., Suto T. 1979; Cellular fatty acid composition in gram-negative obligately anaerobic rods. Journal of General and Applied Microbiology 25:41–51
    [Google Scholar]
  25. Needleman M., Stuchbery P. 1977; The identification of micro-organisms by pyrolysis gas-liquid chromatography. In Analytical Pyrolysis pp. 77–78 Edited by Jones C. E. R., Cramers C. A. Amsterdam: Elsevier;
    [Google Scholar]
  26. Oxborrow G. S., Fields N. D., Puleo J. R. 1976; Preparation of pure microbiological samples for pyrolysis gas-liquid chromatography studies. Applied and Environmental Microbiology 32:302–309
    [Google Scholar]
  27. Quinn P. A. 1974; Development of high resolution pyrolysis gas chromatography for identification of microorganisms. Journal of Chromatographic Science 12:796–806
    [Google Scholar]
  28. Rosebury T. 1962 Microorganisms Indigenous to Man. New York: McGraw-Hill;
    [Google Scholar]
  29. Stack M. V., Donoghue H. D., Tyler J. E. 1978; Discrimination between oral streptococci by pyrolysis gas-liquid chromatography. Applied and Environmental Microbiology 35:45–50
    [Google Scholar]
  30. Sutter V. L., Vargo V. L., Finegold S. M. 1975 Wadsworth Anaerobic Bacteriology Manual, 2nd edn. Los Angeles: The University of California;
    [Google Scholar]
  31. Tydén-Ericsson I. 1973; A new pyrolyser with improved control of pyrolysis conditions. Chromatographia 6:353–358
    [Google Scholar]
  32. Westphal O., Lüderitz O., Bister F. 1952; Über die Extraktion von Bakterienmit Phenol/Wasser. Zeitschrift für Naturforschung 7b:148–155
    [Google Scholar]
  33. Wollenweber H. W., Reitschel E. T., Hofstad T., Weintraub A., Lindberg A. A. 1980; Nature, type of linkage, quantity and absolute configuration of (3-hydroxy) fatty acids in lipopolysaccharides from Bacteroides fragilis NCTC 9343 and related strains. Journal of Bacteriology 144:898–903
    [Google Scholar]
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