RT Journal Article SR Electronic(1) A1 Kistler, H. Corby A1 Vanetten, Hans D.YR 1984 T1 Three Non-allelic Genes for Pisatin Demethylation in the Fungus Nectria haematococca JF Microbiology, VO 130 IS 10 SP 2595 OP 2603 DO https://doi.org/10.1099/00221287-130-10-2595 PB Microbiology Society, SN 1465-2080, AB Isolates of the plant pathogenic fungus Nectria haematococca can demethylate pisatin, an isoflavonoid phytoalexin produced by pea. Previous evidence has shown that pisatin demethylase is a cytochrome P-450 monooxygenase. In culture some isolates can be induced to demethylate pisatin at a high rate by pretreatment with the substrate, while others demethylate pisatin more slowly, regardless of pretreatment. Results from the present study indicate that in two naturally occurring isolates these pisatin demethylating phenotypes are controlled by genes at three loci. The phenotype with high pisatin demethylating activity is conferred by an allele at locus pda-1. Alleles at each of two other loci (pda-2, pda-3) confer low pisatin demethylating activity. Only isolates with negative alleles at all three loci lack pisatin demethylase activity altogether. Locus pda-1 appears to be linked to a gene determining female fertility (fern) in this fungus., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-130-10-2595