1887

Abstract

CBS 14 was grown in batch culture with urea as principal nitrogen source. The lipid content increased from 18% (w/w) with NH -grown cells to 52% (w/w) after 90 h growth. Urea was rapidly taken up and catabolized to release intracellular NH , which accumulated between 10 and 30 h growth. The increase in pool NH content was mirrored by an increase in citric acid accumulation and excretion from the cells. The production of intracellular NH , sufficient to permit lipid accumulation, could be attributed to the increase in activity of urease over this period. Similarly, other catabolic enzymes, such as arginase, threonine dehydratase and NAD: glutamate dehydrogenase, were also induced (or derepressed) when the respective amino acids were used as medium nitrogen source. Growth with mixed organic and inorganic nitrogen compounds considerably decreased the lipid content and was accompanied by a reduction in activity of the various catabolic enzymes concerned. The significance of nitrogen catabolism during lipid accumulation in this yeast is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-7-1705
1984-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/7/mic-130-7-1705.html?itemId=/content/journal/micro/10.1099/00221287-130-7-1705&mimeType=html&fmt=ahah

References

  1. Bender D. A. 1975; Amino Acid Metabolism. London: John Wiley;
    [Google Scholar]
  2. Blinc M., Hočevar B. 1953; Fettanreicherung in Rhodotorula gracilis. Monatshefte für Chemie 84:1127–1131
    [Google Scholar]
  3. Brown C. M. 1980; Ammonia assimilation and utilization in bacteria and fungi. In Microorganisms and Nitrogen Sources pp. 511–535 Edited by Payne J. W. London: John Wiley;
    [Google Scholar]
  4. Brown C. M., Macdonald-Brown D. S., Meers J. L. 1974; Physiological aspects of microbial inorganic nitrogen metabolism. Advances in Microbial Physiology 11:1–52
    [Google Scholar]
  5. Eddy A. A. 1980; Some aspects of amino acid transport in yeast. In Microorganisms and Nitrogen Sources pp. 35–62 Edited by Payne J. W. London: John Wiley;
    [Google Scholar]
  6. Eddy A. A. 1982; Mechanisms of solute transport in selected eukaryotic micro-organisms. Advances in Microbial Physiology 23:1–78
    [Google Scholar]
  7. Evans C. T., Ratledge C. 1984; Effect of nitrogen source on lipid accumulation in oleaginous yeasts. Journal of General Microbiology 130:1693–1704
    [Google Scholar]
  8. Gutmann I., Bergmeyer H. U. 1974; Urea. In Methods of Enzymatic Analysis 4 pp. 1791–1802 Edited by Bergmeyer H. U. New York: Academic Press;
    [Google Scholar]
  9. Habison A., Kubicek C. P., Rohr M. 1979; Phosphofructokinase as a regulatory enzyme in citric acid-producing Aspergillus niger. FEMS Microbiology Letters 5:39–42
    [Google Scholar]
  10. Middelhoven W. J., Hoogkamer-Te Niet M. C. 1981; Repression of catabolic NAD-specific glutamate dehydrogenase of Saccharomyces cerevisiae by arginase, allantoin and urea. FEMS Microbiology Letters 10:307–311
    [Google Scholar]
  11. Pateman J. A., Kinghorn J. R. 1976; Nitrogen metabolism. In The Filamentous Fungi Vol 2, Biosynthesis and Metabolism pp. 159–237 Edited by Smith J. E., Berry D. R. London: Edward Arnold;
    [Google Scholar]
  12. Rohr M., Kubicek C. P. 1981; Regulatory aspects of citric acid fermentation by Aspergillus niger. Process Biochemistry 16:34–37(+44)
    [Google Scholar]
  13. Roon R. J., Levenberg B. 1970; CO2 fixation and the involvement of allophanate in the biotinenzyme-catalyzed cleavage of urea. Journal of Biological Chemistry 245:4593–4595
    [Google Scholar]
  14. Roon R. J., Larimore F., Levy J. S. 1975; Inhibition of amino acid transport by ammonium ion in Saccharomyces cerevisiae. Journal of Bacteriology 124:325–331
    [Google Scholar]
  15. Schimke R. T. 1979; Arginase (rat liver). Methods in Enzymology 17A:313–316
    [Google Scholar]
  16. Shizuta Y., Tokushige M. 1971; Threonine deaminase (degradation) (Escherichia coli). Methods in Enzymology 17B:575–580
    [Google Scholar]
  17. Steiner M. 1959; The utilization of amino and amide nitrogen by Endomycopsis vernalis and other yeasts (with special reference to metabolic reactions at the surface of the cells). Symposia of the Society for Experimental Biology 13:177–192
    [Google Scholar]
  18. Witter B., Debuch H., Steiner M. 1974; Die Lipide von Endomycopsis vernalis bei verschiedener Stickstoff-Ernahrung. Archives of Microbiology 101:321–335
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-7-1705
Loading
/content/journal/micro/10.1099/00221287-130-7-1705
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error