Carbon Utilization by Free-living and Bacteroid Forms of Cowpea Rhiɀobium Strain NGR234 Saroso, S. and Glenn, A. R. and Dilworth, M. J.,, 130, 1809-1814 (1984), doi = https://doi.org/10.1099/00221287-130-7-1809, publicationName = Microbiology Society, issn = 1350-0872, abstract= Free-living cells of the fast-growing cowpea Rhizobium NGR234 were able to grow on a variety of carbon substrates at growth rates varying from 2·5 h on glucose or fumarate to 15·6 h on p-hydroxybenzoate. Free-living cells constitutively oxidized glucose, glutamate and aspartate but were inducible for all the other systems investigated. Bacteroids from root nodules of snake bean, however, were only capable of oxidizing C4-dicarboxylic acids and failed to oxidize any other carbon sources. Free-living cells of NGR234 possess inducible fructose and succinate uptake systems. These substrates are accumulated by active processes since accumulation is inhibited by azide, 2,4-dinitophenol and carbonyl cyanide m-chlorophenyl hydrazone. Bacteroids failed to take up fructose although they actively accumulated succinate, suggesting that the latter substrate is significant in the development of an effective symbiosis., language=, type=