1887

Abstract

Net aerobic H2 production and the induction of uptake hydrogenase activity in the nitrogenfixing Anabaena strains CA and 1F were strictly dependent upon the Ni concentration in the growth medium. Ni concentrations as low as 10 nM blocked H2 production and stimulated an uptake hydrogenase activity in whole cells. Two types of uptake hydrogenase activity were seen: a dark aerobic uptake approximately 30% as active as the H2 production rate, and a lightdependent activity in the presence of DCMU [3-(3,4-dichlorophenyl)-l, 1-dimethylurea] at low oxygen concentrations, amounting to about 50% of the H2 production rate. Together these activities may account for the strong control of net aerobic H2 production by nickel. A significant fraction of the Ni-stimulated uptake hydrogenase activity formed during the transition from nickel deficiency to nickel sufficiency was blocked by chloramphenicol. Nickel may be required for activation of an uptake hydrogenase, or for hydrogenase synthesis, or for synthesis of another protein which is involved in H uptake.

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/content/journal/micro/10.1099/00221287-130-7-1815
1984-07-01
2024-04-24
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References

  1. Bottomley P. J., Van Baalen C. 1978; Dark hexose metabolism by photoautrophically grown cells of the blue-green alga (cyanobacterium) Nostoc sp. strain MAC. Journal of Bacteriology 135:888–894
    [Google Scholar]
  2. Daday A., Smith G. D. 1983; The effect of nickel on the hydrogen metabolism of the cyanobacterium Anabaena cylindrica. . FEMS Microbiology Letters 20:327–330
    [Google Scholar]
  3. Diekert G. B., Graf E.-G., Thauer R. 1979; Nickel requirement for carbon monoxide dehydrogenase formation in Clostridium pasteurianum. . Archives of Microbiology 122:117–120
    [Google Scholar]
  4. Diekert G., Weber B., Thauer R. 1980; Nickel dependence of Factor 430 content in Methanobacterium thermoautotrophicum. . Archives of Microbiology 127:273–278
    [Google Scholar]
  5. Dixon N. E., Gazzola C., Blakeley R. L., Zerner B. 1976; Metal ions in enzymes using ammonia or amides. Science 191:1144–1150
    [Google Scholar]
  6. Eisbrenner G., Roos P., Bothe H. 1981; The number of hydrogenases in cyanobacteria. Journal of General Microbiology 125:383–390
    [Google Scholar]
  7. Ellefson W. L., Whitman W. B., Wolfe R. S. 1982; Nickel-containing factor F430: chromophore of the methylreductase of Methanobacterium. . Proceedings of the National Academy of Sciences of the United States of America 79:3707–3710
    [Google Scholar]
  8. Friedrich C. G., Schneider K., Friedrich B. 1982; Nickel in the catalytically active hydrogenase of Alcaligenes eutrophus. . Journal of Bacteriology 152:42–48
    [Google Scholar]
  9. Gentry C. H. R, Sherrington L. G. 1950; Extraction and photometric estimation of some metals with 8-hydroxyquinoline. Analyst 75:17–21
    [Google Scholar]
  10. Gotto J. W., Tabita F. R., Van Baalen C. 1979; Isolation and characterization of rapidly-growing, marine, nitrogen-fixing strains of blue-green algae. Archives of Microbiology 121:155–159
    [Google Scholar]
  11. Graf E.-G., Thauer R. 1981; Hydrogenase from Methanobacterium thermoautotrophicum, a nickel containing enzyme. FEBS tetters 136:165–169
    [Google Scholar]
  12. Stacey G., Van Baalen C., Tabita F. R. 1977; Isolation and characterization of a marine Anabaena sp. capable of rapid growth on molecular nitrogen. Archives of Microbiology 114:197–201
    [Google Scholar]
  13. Tel-Or E., Luijk L. J., Packer L. 1978; Hydrogenases in N2-fixing cyanobacteria. Archives of Biochemistry and Biophysics 185:185–194
    [Google Scholar]
  14. Van Baalen C. 1962; Studies on marine blue-green algae. Botanica Marina 4:129–139
    [Google Scholar]
  15. Van Baalen C., O’Donnell R. 1978; Isolation of a nickel-dependent blue-green alga. Journal of General Microbiology 105:351–353
    [Google Scholar]
  16. Zhang X., Haskell J. B., Tabita F. R., Van Baalen C. 1983; Aerobic hydrogen production by the heterocystous cyanobacteria, Anabaena spp. A and 1F. Journal of Bacteriology 156:1118–1122
    [Google Scholar]
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