Cyclic GMP and Cyclic AMP Binding Proteins in Myxococcus xanthus Devi, A. L. and McCurdy, H. D.,, 130, 1845-1849 (1984), doi = https://doi.org/10.1099/00221287-130-7-1845, publicationName = Microbiology Society, issn = 1350-0872, abstract= Three binding proteins, one specific for guanosine 3′: 5′-monophosphate (cGMP) and two specific for adenosine 3′: 5′-monophosphate (CAMP) have been partially purified from vegetative cells of Myxococcus xanthus M300. The cGMP binding activity was found in the periplasmic shock fluid. Scatchard analysis indicated only a single class of binding sites with high affinity (apparent K D, 42 nm). The two cAMP binding activities were physically distinct, as indicated by their elution patterns from DEAE-cellulose, K D values and cellular locations. The cytoplasmic cAMP binding protein, which is probably identical to that previously isolated from developing myxospores of M. xanthus had an apparent K D of 57 nm, whereas the periplasmic cAMP binding protein had an apparent KD of 1 μm. During development, the nucleotide binding proteins exhibited changes in activities consistent with their postulated roles during fruiting body development., language=, type=