f Pyoverdine Production by Pseudomonas fluorescens in Synthetic Media with Various Sources of Nitrogen
- Authors: INÉS ALBESA, LUCILA I. BARBERIS, MARÍA C. PÁJARO, ALBERTO J. ERASO
- First Published Online: 01 December 1985, Microbiology 131: 3251-3254, doi: 10.1099/00221287-131-12-3251
- Subject: Biochemistry
- Issue Published:
Summary: The influence of the nitrogen source on pyoverdine production by Pseudomonas fluorescens grown in chemostat culture in synthetic media at constant pH was studied. Pigment synthesis was highest in a medium with proline as the nitrogen source; other amino acids were less effective in promoting pigment synthesis. The pH and Fe3+ content of the media affected pyoverdine production. The ratio of pigment to growth was maximal at pH 8. When the Fe3+ concentration was increased from 10 to 200 μg 1-1 in media with the same nitrogen source, pyoverdine synthesis decreased. Since the Fe3+ contents of the synthetic media were different, the amount present was adjusted to a standard concentration to permit comparisons between the various nitrogen sources.
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