RT Journal Article SR Electronic(1) A1 WHITEHEAD, PHILIP R. A1 BROWN *, NIGEL L.YR 1985 T1 Three Restriction Endonucleases from Anabaena flos-aquae JF Microbiology, VO 131 IS 4 SP 951 OP 958 DO https://doi.org/10.1099/00221287-131-4-951 PB Microbiology Society, SN 1465-2080, AB Three site-specific endonucleases, AflI, AflII and AflIII, have been partially purified from the cyanobacterium Anabaena flos-aquae CCAP 1403/13f. Their recognition and cleavage specificities have been determined to be: A f l I 5 ′ − G − ↓ G ( A T ) − C − C − 3 ′ A f l II 5 ′ − C − ↓ T − T − A − A − G − 3 ′ A f l III 5 ′ − A − ↓ C − Pu − Py − G − T − 3 ′ AflII and AflIII are new specificities and may be useful in molecular cloning, as well as in the analysis of DNA. The distribution of type II restriction endonucleases in the cyanobacteria is briefly discussed., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-131-4-951