1887

Abstract

Summary: Capsular polysaccharide from two strains of serotype T15 was purified and characterized by chemical analysis and NMR spectroscopy. The polymer, a teichoic acid, proved to be very similar in structure to the capsular polysaccharide of serotype T4 and identical to the previously described K62 (K2ab) capsular polysaccharide of . and the capsular polysaccharide of serotype H, i.e. (2-glycerol-3) (phosphate) (4-x-D-galactopyranose-1) with partial -acetylation on the galactose residues. Electron microscopy with Protein A-gold labelled antisera showed that the polysaccharide was peripherally located on the surface of all three organisms. Chemical removal of -acetyl groups from the polysaccharide yielded a structure identical to that previously described for K2 (K2a). Both -acetylated and de--acetylated T15 polymers, when absorbed on to sheep erythroyctes in passive haemagglutination assays, yielded identical antibody titres with sera raised against T15, K 2 or H whole cells. De--acetylation of the Pasteurella polysaccharide influenced its precipitability with immune sera, but this could not be related to the absence of -acetyl groups because the non-acetylated K2 polymer readily precipitated with a line of ‘identity’ with the acetylated T15 polymer.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-8-1963
1985-08-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/8/mic-131-8-1963.html?itemId=/content/journal/micro/10.1099/00221287-131-8-1963&mimeType=html&fmt=ahah

References

  1. Adlam C., Knights J. M., Mugridge A., Lindon J. C., Baker P. R. W., Beesley J. E., Spacey B., Craig G. R., Nagy L. K. 1984; Purification, characterization and immunological properties of the serotype specific capsular polysaccharide of Pasteurella haemolytica (serotype A1) organisms. Journal of General Microbiology 130:2415–2426
    [Google Scholar]
  2. Adlam C., Knights J. M., Mugridge A., Lindon J. C., Williams J. M. 1985; Purification, characterization and immunological properties of the serotype specific capsular polysaccharide of Pasteurella haemolytica (serotype T4) organisms. Journal of General Microbiology 131:387–394
    [Google Scholar]
  3. Axelsen N. H., Krøll J., Week B. 1973; A manual of quantitative immunoelectrophoresis. Scandinavian Journal of Immunology 2: Supplement 1 Oslo: Universitets Forlaget;
    [Google Scholar]
  4. Beesley J. E., Orpin A., Adlam C. 1982; A comparison of immunoferritin, immuno-enzyme and gold-labelled Protein A methods for the localisation of capsular antigen on frozen thin sections of the bacterium Pasteurella haemolytica . Histochemical Journal 14:803–810
    [Google Scholar]
  5. Bodenhausen G., Freeman R. 1977; Correlation of proton and carbon-13 spectra by heteronuclear two-dimensional spectroscopy. Journal of Magnetic Resonance 28:471–476
    [Google Scholar]
  6. Fischer W., Schmidt M. A., Jann B., Jann K. 1982; Structure of the Escherichia coli K2 capsular antigen. Stereochemical configuration of the glycerophosphate and distribution of galactopyranosyl and galactofuranosyl residues. Biochemistry 21:1279–1284
    [Google Scholar]
  7. Gilmour N. J. L. 1980; Pasteurella haemolytica infections in sheep. Veterinary Quarterly 2:191–197
    [Google Scholar]
  8. Jann K., Schmidt M. A. 1980; Comparative chemical analysis of two variants of the Escherichia coli K2 antigen. FEMS Microbiology Letters 7:79–81
    [Google Scholar]
  9. Jann K., Jann B., Schmidt M. A., Vann W. F. 1980; Structure of the Escherichia coli K2 capsular antigen, a teichoic acid-like polymer. Journal of Bacteriology 143:1108–1115
    [Google Scholar]
  10. Kurz G., Wallenfels K. 1974; d-Galactose UV-assay with galactose dehydrogenase. In Methoden der Enzymatischen Analyse 3rd edn, vol II pp 1279–1282 Edited by Bergmeyer H. U. Stuttgart: Verlag Chemie;
    [Google Scholar]
  11. Larsen J. C., Orskov F., Orskov I., Schmidt M. A., Jann B., Jann K. 1980; Crossed immunoelectrophoresis and chemical structural analysis used for characterization of two varieties of Escherichia coli K2 polysaccharide antigen. Medical Microbiology and Immunology 168:191–200
    [Google Scholar]
  12. Michon F., Roy R., Jennings H. J., Ashton F. E. 1984; Structural elucidation of the capsular polysaccharide of Neisseria meningitidis group H. Canadian Journal of Chemistry 62:1519–1524
    [Google Scholar]
  13. Poxton I. R., Tarelli E., Baddiley J. 1978; The structure of C-polysaccharide from the walls of Streptococcus pneumoniae . Biochemical Journal 175:1033–1042
    [Google Scholar]
  14. Van der Kaaden A., Van doorn-Van Wakeren J. I. M., Kamerling J. P., Vliegenthart J. F. G., Tiesjema R. H. 1984; Structure of the capsular antigen of Neisseria meningitidis serogroup H. European Journal of Biochemistry 141:513–519
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-8-1963
Loading
/content/journal/micro/10.1099/00221287-131-8-1963
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error