f The Permeability Parameter of the Outer Membrane of Pseudomonas aeruginosa Varies with the Concentration of a Test Substrate, Cephalosporin C
- Authors: R. GLYN HEWINSON, DAVID C. LANE, MARY P. E. SLACK, WRIGHT W. NICHOLS
- Microbiology, January 1986 132: 27-33, doi: 10.1099/00221287-132-1-27
- Subject: Physiology And Growth
- Published Online:
SUMMARY: The permeability parameter (C) for the movement of cephalosporin C across the outer membrane of Pseudomonas aeruginosa was measured using the widely accepted method of Zimmermann & Rosselet. In one experiment, the value of C varied continuously from 4·2 to 10·8 cm3 min-1 (mg dry wt cells)-1 over a range of concentrations of the test substrate, cephalosporin C, from 50 to 5 μm. Dependence of C on the concentration of test substrate was still observed when the effect of a possible electric potential difference across the outer membrane was corrected for. In quantitative studies of β-lactam permeation the dependence of C on the concentration of β-lactam should be taken into account.
© Society for General Microbiology 1986 | Published by the Microbiology Society
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