1887

Abstract

Summary: In sugar-grown cells of cowpea strain NGR234 activities for enzymes of the Entner-Doudoroff and pentose phosphate pathways were present while the virtual absence of phospho-fructokinase and fructose-bisphosphate aldolase indicated that the Embden–Meyerhof–Parnas pathway was unlikely to be significant. Invertase, fructokinase, glucose-6-phosphate dehydrogenase and the Entner–Doudoroff enzymes were present at only low activities in succinate grown cells, but were induced in sugar-grown cells. Isolated snakebean bacteroids contained very low activities of these four enzymes. Although C-dicarboxylic acids exerted some repressive effect on induction of these enzymes, there was substantial enzyme activity induced in cells grown on sucrose plus a C dicarboxylic acid. The data suggest that the peribacteroid membrane may be relatively impermeable to sugars and so dictate the carbon source(s) available to the bacteroids.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-2-243
1986-02-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/2/mic-132-2-243.html?itemId=/content/journal/micro/10.1099/00221287-132-2-243&mimeType=html&fmt=ahah

References

  1. Allison W. S., Kaplan N. O. 1964; The comparative enzymology of triosephosphate dehydrogenase. Journal of Biological Chemistry 239:2140–2152
    [Google Scholar]
  2. Arwas R., McKay I. A., Rowney F. R. P., Dilworth M. J., Glenn A. R. 1985; Properties of organic acid utilization mutants of Rhizobium leguminosarum strain 300. Journal of General Microbiology 131:2059–2066
    [Google Scholar]
  3. Broughton W. J., Dilworth M. J. 1971; Control of leghaemoglobin synthesis in snake beans. Biochemical Journal 125:1075–1080
    [Google Scholar]
  4. Brown C. M., Dilworth M. J. . 1975; Ammonia assimilation by Rhizobium cultures and bacteroids. Journal of General Microbiology 86:39–48
    [Google Scholar]
  5. Bucher J., Pfleiderer G. 1955; Pyruvate kinase from muscle. Methods in Enzymology 1:435–440
    [Google Scholar]
  6. Cha S. 1969; Succinate thiokinase from pig heart. Methods in Enzymology 13:62–69
    [Google Scholar]
  7. Dilworth M. J., Glenn A. R. 1981; Control of carbon substrate utilization by rhizobia. Edited by A. H. Gibson & W. E. Newton, Canberra, Australia: Australian Academy of Science. In Current Perspectives in Nitrogen Fixation, pp.244–256
    [Google Scholar]
  8. Dilworth M. J., Glenn A. R. 1984; Transport in Rhizobium and its significance to the legume symbiosis. Edited by P. W. Ludden & J. E. Burris. New York: Elsevier. In Nitrogen Fixation and CO2 Fixation, pp.53–61
    [Google Scholar]
  9. Finan T. M., Wood J. M., Jordan D. C. 1983; Symbiotic properties of C4-dicarboxylic acid transport mutants of Rhizobium leguminosarum. Journal of Bacteriology 154:1403–1413
    [Google Scholar]
  10. Glenn A. R., Brewin N. J. 1981; Succinate-resistant mutants of Rhizobium leguminosarum. Journal of General Microbiology 126:237–241
    [Google Scholar]
  11. Glenn A. R., Dilworth M. J. 1981; The uptake and hydrolysis of disaccharides by fast- and slow-growing species of Rhizobium. Archives of Microbiology 129:233–239
    [Google Scholar]
  12. Glenn A. R., Poole P. S., Hudman J. F. 1980; Succinate uptake by free-living and bacteroid forms of Rhizobium leguminosarum. Journal of General Microbiology 119:267–271
    [Google Scholar]
  13. Glenn A. R., McKay I. A., Arwas R., Dilworth M. J. 1984; Sugar metabolism and the symbiotic properties of carbohydrate mutants of Rhizobium leguminosarum. Journal of General Microbiology 130:239–245
    [Google Scholar]
  14. Hederstedt L., Holmgren E., Rugberg L. 1979; Characterization of a succinate dehydrogenase complex solubilized from the cytoplasmic membrane of Bacillus subtilis with the nonionic detergent Triton X-100. Journal of Bacteriology 138:370–376
    [Google Scholar]
  15. Hill R. L., Bradshaw R. A. 1969; Fumarase. Methods in Enzymology 13:91–99
    [Google Scholar]
  16. Keele B. B., Hamilton P. B., Elkan G. H. 1969; Glucose catabolism in Rhizobium japonicum. Journal of Bacteriology 97:1184–1191
    [Google Scholar]
  17. Krebs A., Bridger W. A. 1980; The kinetic properties of phosphoenolpyruvate carboxykinase of Escherichia coli. Canadian Journal of Biochemistry 58:309–318
    [Google Scholar]
  18. Lynch W. H., MacLeod J., Franklin M. 1975; Effect of temperature on the activity and synthesis of glucose-catabolizing enzymes in Pseudomonas fluorescens. Canadian Journal of Microbiology 21:1560–1572
    [Google Scholar]
  19. McKay I. A., Glenn A. R., Dilworth M. J. 1985; Gluconeogensis in Rhizobium leguminosarum MNF3841. Journal of General Microbiology 131:2067–2073
    [Google Scholar]
  20. Mieyal J. J. 1972; Sucrose phosphorylase from Pseudomonas saccharophila. . Methods in Enzymology 28:935–943
    [Google Scholar]
  21. Mulongoy K., Elkan G. H. 1977; Glucose catabolism in two derivatives of a Rhizobium japonicum strain differing in nitrogen-fixing efficiency. Journal of Bacteriology 131:179–187
    [Google Scholar]
  22. Novello F., McLean G. 1968; The pentose phosphate pathway of glucose metabolism. Biochemical Journal 107:775–791
    [Google Scholar]
  23. Ochoa S. 1955; Isocitrate dehydrogenase system (TPN) from pig heart. Methods in Enzymology 1:699–704
    [Google Scholar]
  24. Reeves H. C., Rabin R., Wegener W. S., Ajl S. J. 1971; Assays of enzymes of the tricarboxylic acid and glyoxylate cycles. Methods in Microbiology 6A:425–462
    [Google Scholar]
  25. Reibach P. H., Benedict C. R. 1977; Fractionation of stable carbon isotopes by phosphoenolpyruvate carboxylase from C4 plants. Plant Physiology 59:564–568
    [Google Scholar]
  26. Reibach P. H., Streeter J. G. 1984; Evaluation of active versus passive uptake of metabolites by Rhizobium japonicum bacteroids. Journal of Bacteriology 159:47–52
    [Google Scholar]
  27. Reibach P. H., Mask P. L., Streeter J. G. 1981; A rapid one-step method for the isolation of bacteroids from root nodules of soybean plants, utilizing self-generating Percoll gradients. Canadian Journal of Microbiology 27:491–495
    [Google Scholar]
  28. Ronson C. W., Primrose S. B. 1979; Carbohydrate metabolism in Rhizobium trifolii: identification and symbiotic properties of mutants. Journal of General Microbiology 112:77–88
    [Google Scholar]
  29. Ronson C. W., Lyttleton P., Robertson J. G. 1981; C4-Dicarboxylate transport mutants of Rhizobium trifolii form ineffective nodules on Trifolium repens . Proceedings of the National Academy of Sciences of the United States of America 78:4284–4288
    [Google Scholar]
  30. Saroso S., Glenn A. R., Dilworth M. J. 1984; Carbon utilization by free-living and bacteroid forms of cowpea Rhizobium strain NGR234. Journal of General Microbiology 130:1809–1814
    [Google Scholar]
  31. Stowers M. D., Elkan G. H. 1983; The transport and metabolism of glucose in cowpea rhizobia. Canadian Journal of Microbiology 29:398–406
    [Google Scholar]
  32. Suzuki K., Imahori K. 1982; Phosphoglycerate kinase from Bacillus stearothermophilus. . Methods in Enzymology 90:126–130
    [Google Scholar]
  33. Trinder P. 1969; Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Annals of Clinical Biochemistry 6:24–27
    [Google Scholar]
  34. Ucker D. S., Signer E. R. 1978; Cataboliterepression-like phenomenon in Rhizobium meliloti. . Journal of Bacteriology 136:1197–1200
    [Google Scholar]
  35. Vincent J. M. 1970; A Manualfor the Practical Study of the Root Nodule Bacteria. Oxford: Blackwell Scientific Publications.
    [Google Scholar]
  36. Von Tigerstrom M., Campbell J. J. R. 1966; The tricarboxylic acid cycle, the glyoxylate cycle, and the enzymes of glucose oxidation in Pseudomonas aeruginosa. Canadian Journal of Microbiology 12:1015–1022
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-2-243
Loading
/content/journal/micro/10.1099/00221287-132-2-243
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error