The Use of Activities of Carbon Catabolic Enzymes as a Probe for the Carbon Nutrition of Snakebean Nodule Bacteroids
Saroso, S. and Dilworth, M. J. and Glenn, A. R.,, 132, 243-249 (1986),
doi = https://doi.org/10.1099/00221287-132-2-243,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= Summary: In sugar-grown cells of cowpea Rhizobium strain NGR234 activities for enzymes of the Entner-Doudoroff and pentose phosphate pathways were present while the virtual absence of phospho-fructokinase and fructose-bisphosphate aldolase indicated that the Embden–Meyerhof–Parnas pathway was unlikely to be significant. Invertase, fructokinase, glucose-6-phosphate dehydrogenase and the Entner–Doudoroff enzymes were present at only low activities in succinate grown cells, but were induced in sugar-grown cells. Isolated snakebean bacteroids contained very low activities of these four enzymes. Although C4-dicarboxylic acids exerted some repressive effect on induction of these enzymes, there was substantial enzyme activity induced in cells grown on sucrose plus a C4 dicarboxylic acid. The data suggest that the peribacteroid membrane may be relatively impermeable to sugars and so dictate the carbon source(s) available to the bacteroids.,
language=,
type=