1887

Microbiology Society logo

Volume 132, Issue 2

Genetic Heterogeneity among Isolates of Free

Abstract

Summary: Chromosomal and plasmid DNA have been extracted from six isolates of , the aetiological agent of Q fever. Restriction fragment length polymorphisms detected after III digestions of chromosomal DNA revealed four different patterns that distinguished the American from the European isolates, and the Nine Mile phase I prototype strain from a spontaneously derived, isogenic phase II nonrevertant variant. At least one of the III fragments visible in the pattern from Nine Mile phase I and not in that from Nine Mile phase II could not be detected by DNA–DNA hybridization, and thus may have been deleted during the phase transition. Comparison of Nine Mile phase II, which does not survive animal passage, with Grita M44 phase II, which does, indicated that the III fragment was present in the Grita strain. These results suggest that this III fragment may be concerned with functions necessary to survive the cellular immune response Isolates from two human endocarditis cases showed the greatest divergence from all the other isolates, having at least five fragments of unique mobility in the III digestion pattern of their chromosomal DNA. Also, a plasmid obtained from these two isolates was 2 to 3 kb larger than the plasmid present in the other five isolates, and its restriction pattern could be distinguished from that of the other plasmids by several endonucleases. Detection of chromosomal and plasmid restriction fragment length polymorphisms among strains of phase I or phase II from various geographical locations and environmental sources will facilitate Q fever diagnosis and strain identification.

  • Received:
  • Published Online:
© Society for General Microbiology 1986
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-2-455
1986-02-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/2/mic-132-2-455.html?itemId=/content/journal/micro/10.1099/00221287-132-2-455&mimeType=html&fmt=ahah

References

  1. Amano K.-I., Williams J. C. 1984; Chemical and immunological characterization of lipopolysaccharides from phase I and phase II Coxiella burnetii. . Journal of Bacteriology 160:994–1002
     [Google Scholar]
  2. Babudieri B. 1953; Q fever: a zoonosis. Advances in Veterinary Science 5:81–154
     [Google Scholar]
  3. Benson W. W., Brook D. W., Mather J. 1963; Serologic analysis of a penitentiary group using raw milk from a Q fever infected herd. Public Health Report 78:707–710
     [Google Scholar]
  4. Biberstein E. L., Behymer D. E., Bushnell R., Crenshaw G., Riemann H. P., Franti C. E. 1974; A survey of Q fever (Coxiella burnetii) in Californian dairy cows. American Journal of Veterinary Research 35:1577–1582
     [Google Scholar]
  5. Burnet F. M., Freeman M. 1937; Experimental studies on virus of ‘Q’ fever. Medical Journal of Australia 2:299–305
     [Google Scholar]
  6. Burton P. R., Stueckemann J., Welsh R. M., Paretsky D. 1978; Some ultrastructural effects of persistent infections by the rickettsia Coxiella burnetii in mouse L cells and green monkey kidney (Vero) cells. . Infection and Immunity 21:556–566
     [Google Scholar]
  7. Cox H. R. 1939; Studies of filter-passing infectious agent isolated from ticks; further attempts to cultivate in cell-free media; suggested classification. Public Health Report 54:1822–1827
     [Google Scholar]
  8. Cox H. R., Bell E. J. 1939; Cultivation of Rickettsia diaporica in tissue culture and in tissues of developing chick embryos. Public Health Report 54:2171–2178
     [Google Scholar]
  9. Davis G. E., Cox H. R. 1938; Filter-passing infectious agent isolated from ticks; isolation from Dermacentor andersoni, reactions in animals, and filtration experiments. Public Health Report 53:2259–2267
     [Google Scholar]
  10. DeLay P. D., Lennette E. H., De Ome K. B. 1950; Q fever in California; recovery of Coxiella burnetii from naturally-infected air-borne dust. Journal of Immunology 65:211–220
     [Google Scholar]
  11. Derrick E. H. 1937; ‘Q’ fever, a new fever entity: clinical features, diagnosis and laboratory investigation. Medical Journal of Australia 2:281–299
     [Google Scholar]
  12. Derrick E. H., Smith D. J. W. 1940; Studies in epidemiology of Q fever; the isolation of 3 strains of Rickettsia burnetii from the bandicoot Isoodon toro-sus. Australian Journal of Experimental Biology and Medical Science 18:99–102
     [Google Scholar]
  13. Genig V. A. 1960; Attenuated variant ‘M’ of Rickettsia burnetii as a possible live vaccine against Q-fever. Vestnik Akademii meditsinskikh nauk SSSR 2:46–57
     [Google Scholar]
  14. Goldman R. C., Leive L. 1980; Heterogeneity of antigens - side chain length in lipopolysaccharide from Escherichia coli O111 and Salmonella typhimurium LT2. European Journal of Biochemistry 107:145–153
     [Google Scholar]
  15. Hackstadt T., Williams J. C. 1981; Biochemical stratagem for obligate parasitism of eukaryotic cells by Coxiella burnetii. Proceedings of the National Academy of Sciences of the United States of America 78:3240–3244
     [Google Scholar]
  16. Johnson J. W., Eddy G. A., Pedersen C. E. Jr. 1976; Biological properties of the M-44 strain of Coxiella burneti. Journal of Infectious Diseases 133:334–338
     [Google Scholar]
  17. Kazar J., Brezina R., Schramek S., Urvolgyi J., Pospisil V., Kovacova E. 1974; Virulence, antigenic properties and physio-chemical characteristics of Coxiella burnetii strains with different chick embryo yolk sac passage history. Acta virologica 18:434–442
     [Google Scholar]
  18. Kimbrough R. C., Ormsbee R. A., Peacock M., Rogers W. R., Bennetts R. W., Raaf J., Krause A., Gardner C. 1979; Q fever endocarditis in the United States. Annals of Internal Medicine 91:400–402
     [Google Scholar]
  19. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
    [Google Scholar]
  20. Marmion B. P., Stoker M. G. P. 1950; Q fever in Great Britain; epidemiology of an outbreak. Lancet ii:611–616
     [Google Scholar]
  21. Marshak R. R., Melbin J., Herman M. J. 1961; Study of Q fever in animals and man in Pennsylvania. American Journal of Public Health 51:1189–1198
     [Google Scholar]
  22. O’Rourke A. T., Samuel J. E., Natvig D. O., Frazier M. E., Mallavia L. P., Bacca O. 1984; Restriction endonuclease analysis of phase I and phase II Coxiella burnetii DNA. In Abstracts of the Annual Meeting of the American Society for Microbiology, D67, p. 62. Edited by F. C. Neidhardt. Washington, DC: American Society for Microbiology.
    [Google Scholar]
  23. Peacock M. G., Philip R. N., Williams J. C., Faulkner R. S. 1983; Serological evaluation of Q fever in humans: enhanced phase I titers of immunoglobulins G and A are diagnostic for Q fever endocarditis. Infection and Immunity 41:1089–1098
     [Google Scholar]
  24. Reed C. F., Wentworth B. B. 1957; Q fever studies in Ohio. Journal of the American Veterinary Medical Association 130:458–1098
     [Google Scholar]
  25. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
     [Google Scholar]
  26. Robbins F. C., Rustigian R., Snyder M. J., Smadel J. E. 1946; Q fever in the Mediterranean area: report of its occurrence in allied troops. III. The etiological agent. American Journal of Hygiene 44:51–63
     [Google Scholar]
  27. Samuel J. E., Frazier M. E., Kahn M. L., Smadel J. E., Thomashow L. S., Mallavia L. P. 1983; Isolation and characterization of a plasmid from phase I Coxiella burnetii. Infection and Immunity 41:488–493
     [Google Scholar]
  28. Schramek S., Mayer H. 1982; Different sugar compositions of lipopolysaccharides isolated from phase I and phase II cells of Coxiella burnetii. Infection and Immunity 38:53–57
     [Google Scholar]
  29. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
     [Google Scholar]
  30. Stoker M. G. P. , Fiset P. 1956; Phase variation of the Nine Mile and other strains of Rickettsia burnetii. Canadian Journal of Microbiology 2:310–321
     [Google Scholar]
  31. Williams J. C. , Stewart S. 1984; Identification of immunogenic proteins of Coxiella burnetii phase variants. Edited by L. Leive & D. Schlessinger. Washington, DC: American Society for Microbiology. In Microbiology - 1984 pp:257–262
     [Google Scholar]
  32. Williams J. C. , Peacock M. G., McCaul T. F. 1981; Immunological and biological characterization of Coxiella burnetii, phases I and II, separated from host components. Infection and Immunity 32:840–851
     [Google Scholar]
  33. Williams J. C. , Johnston M. R., Peacock M. G., Thomas L. A., Stewart S., Portis J. L. 1984; Monoclonal antibodies distinguish phase variants of Coxiella burnetii. Infection and Immunity 43:421–428
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-2-455
Loading
Genetic Heterogeneity among Isolates of Coxiella burnetii
Microbiology 132, 455 (1986); https://doi.org/10.1099/00221287-132-2-455
/content/journal/micro/10.1099/00221287-132-2-455
/content/journal/micro/10.1099/00221287-132-2-455
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error