1887

Abstract

Summary: Colonial variants of strain P9 expressing different pili and/or outer membrane protein II (P.II) were investigated with respect to their interaction with human polymorphonuclear leucocytes (PMN). Two assay systems were used. A phagocytic killing assay measured the intracellular survival of gonococci, and PMN chemiluminescence (CL) was used to determine the initial surface interactions. All variants expressing P.II were killed effectively by PMN and also greatly stimulated PMN CL. The P.II variants, on the other hand, were resistant to phagocytic killing and stimulated a much lower CL response. The presence of different P.II species was associated with different CL profiles and therefore different modes of interaction with the PMN membrane. A P.II-specific monoclonal IgG was opsonic and greatly increased PMN CL in contrast to F(abʹ) prepared from the same antibody, which inhibited it, thus confirming the role of P.II in the PMN interaction. Phagocytic killing assays revealed that with the loss of P.II, gonococcal variants acquired resistance to killing. Comparison of piliated and non-piliated pairs of variants with the same P.II profile showed that PMN–gonococcal interactions are dominated by the nature of the P.II species present whereas pili have little effect.

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1986-02-01
2024-03-28
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References

  1. Allen R. C., Loose L. D. 1976; Phagocytic activation of a luminol-dependent chemiluminescence in rabbit alveolar and peritoneal macrophages. Biochemical and Biophysical Research Communications 69:245–252
    [Google Scholar]
  2. Allen R. C., Stjernholm R. L., Steele R. H. 1972; Evidence for the generation of (an) electronic excitation state(s) in human polymorphonuclear leukocytes and its participation in bactericidal activity. Biochemical and Biophysical Research Communications 47:679–684
    [Google Scholar]
  3. Davis A. T., Estensen R., Quie P. G. 1971; Cytochalasin B. III. Inhibition of human polymorphonuclear leukocyte phagocytosis. Proceedings of the Society for Experimental Biology and Medicine 137:161–164
    [Google Scholar]
  4. Densen P., Mandell G. L. 1978; Gonococcal interactions with polymorphonuclear neutrophils: importance of the phagosome for bactericidal activity. Journal of Clinical Investigation 62:1161–1171
    [Google Scholar]
  5. Diaz J.-L., Heckels J. E. 1982; Antigenic variation of outer membrane protein II in colonial variants of Neisseria gonorrhoeae P9. Journal of General Microbiology 128:585–591
    [Google Scholar]
  6. Dilworth J. A., Hendley J. O., Mandell G. L. 1975; Attachment and ingestion of gonococci by human neutrophils. Infection and Immunity 11:512–516
    [Google Scholar]
  7. Gibbs D. L., Roberts R. B. 1975; The interaction in vitro between human polymorphonuclear leukocytes and Neisseria gonorrhoeae cultivated in the chick embryo. Journal of Experimental Medicine 141:155–171
    [Google Scholar]
  8. Goldstein I. M., Roos D., Kaplan H. B., Weissmann G. 1975; Complement and immunoglobulins stimulate superoxide production by human leukocytes independently of phagocytosis. Journal of Clinical Investigation 56:1155–1163
    [Google Scholar]
  9. Grebner J. V., Mills E. L., Gray B. H., Quie P. G. 1977; Comparison of phagocytic and chemiluminescence response of human polymorphonuclear neutrophils. Journal of Laboratory and Clinical Medicine 89:153–159
    [Google Scholar]
  10. Heckels J. E. 1982; Role of surface proteins in the adhesion of Neisseria gonorrhoeae. In Microbiology 1982, pp. 301–304 Edited by D. Schlessinger. Washington, DC: American Society for Microbiology.
    [Google Scholar]
  11. James J. F., Swanson J. 1978; Studies on gonococcus infection. XIII. Occurrence of color/opacity colonial variants in clinical cultures. Infection and Immunity 19:332–340
    [Google Scholar]
  12. James J. F., Lammel C. J., Draper D. L., Brooks G. F. 1980; Attachment of N. gonorrhoeae colony phenotype variants to eukaryotic cells and tissues. In Genetics and Immunobiology of Pathogenic Neisseria, pp. 213–216 Edited by D. Danielsson & S. Normark. Sweden: University of Umėa, UmSa.
    [Google Scholar]
  13. Lambden P. R. 1982; Biochemical comparison of pili from variants of Neisseria gonorrhoeae P9. Journal of General Microbiology 128:2105–2111
    [Google Scholar]
  14. Lambden P. R., Heckels J. E. 1979; Outer membrane protein composition and colonial morphology of Neisseria gonorrhoeae strain P9. FEMS Microbiology Letters 5:263–265
    [Google Scholar]
  15. Lambden P. R., Heckels J. E., James L. T., Watt P. J. 1979; Variations in surface protein composition associated with virulence properties in opacity types of Neisseria gonorrhoeae. Journal of General Microbiology 114:305–312
    [Google Scholar]
  16. Lambden P. R., Heckels J. E., McBride H., Watt P. J. 1981; The identification and isolation of novel pilus types produced by variants of N. gonorrhoeae P9 following selection in vivo. FEMS Microbiology Letters 10:339–341
    [Google Scholar]
  17. Magnusson K. E., Kihlstrom E., Norlander L., Norquist A., Davies J., Normark S. 1979; Effect of colony type and pH on surface charge and hydrophobicity of Neisseria gonorrhoeae. Infection and Immunity 26:397–401
    [Google Scholar]
  18. Mardh P. A., Westrom L. 1976; Adherence of bacteria to vaginal epithelial cells. Infection and Immunity 13:661–666
    [Google Scholar]
  19. McBride H. M., Lambden P. R., Heckels J. E., Watt P. J. 1981; The role of outer membrane proteins in the survival of Neisseria gonorrhoeae P9 within guinea-pig subcutaneous chambers. Journal of General Microbiology 126:63–67
    [Google Scholar]
  20. Okuda K. 1975; Effects of Cytochalasin B on the intracellular bactericidal activity of human neutrophils. Antimicrobial Agents and Chemotherapy 7:736–741
    [Google Scholar]
  21. Punsalang A. P., Sawyer W. D. 1973; Role of pili in the virulence of Neisseria gonorrhoeae. Infection and Immunity 8:255–263
    [Google Scholar]
  22. Repine J. E., Johansen K. S., Berger E. M. 1984; Hydroxyl radical scavengers produce similar decreases in the chemiluminescence responses and bactericidal activities of neutrophils. Infection and Immunity 43:435–437
    [Google Scholar]
  23. Rest R. F., Fischer S. H., Ingham Z. Z., Jones J. F. 1982; Interactions of Neisseria gonorrhoeae with human neutrophils: effects of serum and gonococcal opacity on phagocyte killing and chemiluminescence. Infection and Immunity 36:737–744
    [Google Scholar]
  24. Robinson P., Wakefield D., Breit S. N., Easter J. F., Penny R. 1984; Chemiluminescent response to pathogenic organisms: normal human polymorphonuclear leukocytes. Infection and Immunity 43:744–752
    [Google Scholar]
  25. Rothbard J. B., Fernandez R., Schoolnik G. K. 1984; Strain-specific and common epitopes of gonococcal pili. Journal of Experimental Medicine 160:208–221
    [Google Scholar]
  26. Swanson J. 1978; Studies on gonococcus infection. XIV. Cell wall protein differences among color/opacity colony variants of Neisseria gonorrhoeae. Infection and Immunity 21:292–302
    [Google Scholar]
  27. Swanson J., Heckels J. E. 1980; Proposal: nomenclature of gonococcal outer membrane proteins. In Genetics and Immunobiology of Pathogenic Neisseria, pp. xxi–xxii Edited by D. Danielsson & S. Normark. Sweden: University of Umėa, Umėa.
    [Google Scholar]
  28. Swanson J., King G. 1978; Neisseria gonorrhoeae-granulocyte interactions. In Immunobiology of Neisseria gonorrhoeae, pp. 221–226 Edited by G. F. Brooks, E. C. Gotschlich, K. K. Holmes, W. D. Sawyer & F. E. Young. Washington, DC: American Society for Microbiology.
    [Google Scholar]
  29. Swanson J., Sparks E., Young D., King G. 1975; Studies on gonococcus infection. X. Pili and leukocyte association factor as mediators of interactions between gonococci and eukaryotic cells in vitro. Infection and Immunity 11:1352–1361
    [Google Scholar]
  30. Thomas D. W., Hill J. C., Tyeryar F. J., Jr. 1973; Interaction of gonococci with phagocytic leukocytes from men and mice. Infection and Immunity 8:98–104
    [Google Scholar]
  31. Trust T. J., Lambden P. R., Watt P. J. 1980; The cohesive properties of variants of Neisseria gonorrhoeae strain P9: specific pilus-mediated and non-specific interactions. Journal of General Microbiology 119:179–187
    [Google Scholar]
  32. Virji M., Everson J. S. 1981; Comparative virulence of opacity variants of Neisseria gonorrhoeae strain P9. Infection and Immunity 319:965–970
    [Google Scholar]
  33. Virji M., Heckels J. E. 1983; Antigenic cross-reactivity of Neisseria pili: investigations with type-and species-specific monoclonal antibodies. Journal of General Microbiology 129:2761–2768
    [Google Scholar]
  34. Virji M., Heckels J. E. 1985; Role of anti-pilus antibodies in host defense against gonococcal infection studied with monoclonal anti-pilus antibodies. Infection and Immunity 49:621–628
    [Google Scholar]
  35. Virji M., Heckels J. E., Watt P. J. 1983; Monoclonal antibodies to gonococcal pili: studies on antigenic determinants on pili from variants of strain P9. Journal of General Microbiology 129:1965–1973
    [Google Scholar]
  36. Ward M. E., Watt P. J., Robertson J. N. 1974; The human fallopian tube: a laboratory model for gonococcal infection. Journal of Infectious Diseases 129:650–659
    [Google Scholar]
  37. Witt K., Veale D. R., Finch H., Penn C. W., Sen D., Smith H. 1976; Resistance of Neisseria gonorrhoeae grown in vivo to ingestion and digestion by phagocytes of human blood. Journal of General Microbiology 96:341–350
    [Google Scholar]
  38. Zak K., Diaz J.-L., Jackson D., Heckels J. E. 1984; Antigenic variation during infection with Neisseria gonorrhoeae: detection of antibodies to surface proteins in sera of patients with gonorrhea. Journal of Infectious Diseases 149:166–173
    [Google Scholar]
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