1887

Abstract

Summary: Two DNA fragments, one encoding the promoter and the other encoding a sequence from the early region of phage SPO1, were cloned into the promoter-probe vector pPL603. Both fragments effected strong promoter activity in vegetative cells.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-2-565
1986-02-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/2/mic-132-2-565.html?itemId=/content/journal/micro/10.1099/00221287-132-2-565&mimeType=html&fmt=ahah

References

  1. Bolivar F., Backman K. 1979; Plasmids of Escherichia coli as cloning vectors. Methods in Enzymology 68:245–280
    [Google Scholar]
  2. Brosius J., Erfle M., Storella J. 1985; Spacing of the -10 and 35 regions in the tac promoter. Journal of Biological Chemistry 260:3539–3541
    [Google Scholar]
  3. Gryczan T., Contente S., Dubnau D. 1980; Molecular cloning of heterologous chromosomal DNA by recombination between a plasmid vector and a homologous resident plasmid. Molecular and General Genetics 177:459–467
    [Google Scholar]
  4. Keggins K. M., Lovett P. S., Duvall E. J. 1978; Molecular cloning of genetically active fragments of Bacillus DNA in Bacillus subtilis and properties of the vector plasmid pUBllO. Proceedings of the National Academy of Sciences of the United States of America 75:1423–1427
    [Google Scholar]
  5. Lee G., Pero J. 1981; Conserved nucleotide sequences in temporally controlled bacteriophage promoters. Journal of Molecular Biology 151:247–265
    [Google Scholar]
  6. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning, a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
    [Google Scholar]
  7. Maxam A. M., Gilbert W. 1980; Sequencing end-labelled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  8. Moran C. P., Lang N., LeGrice S. F. J., Lee G., Stephans M., Sonenshein A. L., Pero J., Losick R. 1982; Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Molecular and General Genetics 186, 339-346.
    [Google Scholar]
  9. Osburne M. S., Craig R. J., Rothstein D. M. 1985; A thermoinducible transcription system for B. subtilis utilizing control elements from temperate phage ф 105. Journal of Bacteriology 163:1101–1108
    [Google Scholar]
  10. Shapiro J. A., Dean D. H., Halvorson H. O. 1974; Low-frequency specialized transduction with the Bacillus subtilis bacteriophage ф 105. Virology 62:393–403
    [Google Scholar]
  11. Shaw W. N. 1975; Chloramphenicol acetyltransfer-ase from chloramphenicol-resistant bacteria. Methods in Enzymology 43:737–755
    [Google Scholar]
  12. Sonenshein A. L., Cami B., Brevet J., Cote R. 1974; Isolation and characterization of rifampin-resistant and streptolydigin-resistant mutants of Bacillus subtilis with altered sporulation properties. Journal of Bacteriology 120:253–265
    [Google Scholar]
  13. Williams D. M., Duvall E. J., Lovett P. J. 1981; Cloning restriction fragments that promote expression of a gene in Bacillus subtilis. . Journal of Bacteriology 146:1162–1165
    [Google Scholar]
  14. Yansura D. G., Henner D. J. 1984; Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis. . Proceedings of the National Academy of Sciences of the United States of America 81:439–443
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-2-565
Loading
/content/journal/micro/10.1099/00221287-132-2-565
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error