1887

Abstract

Summary: Stationary phase cells of can form either a bud or a hypha, depending upon the pH of the medium into which they are released. At low pH, cells form an ellipsoidal bud and at high pH, cells form an elongated hypha. By staining cells with rhodamine-conjugated phalloidin, we have compared the dynamics of actin localization during the formation of buds and hyphae. Before evagination, actin granules were distributed throughout the cytoplasmic cortex in both budding and hypha-forming cells. Just before evagination, actin granules clustered at the site of evagination, then filled the early evagination in both budding and hypha-forming cells. With continued bud growth, the actin granules then redistributed throughout the cytoplasmic cortex. In marked contrast, with continued hyphal growth, the majority of actin granules clustered at the hyphal apex. This distinct difference in actin granule localization may be related to the distinct differences in the expansion zones of the cell wall recently demonstrated between growing buds and hyphae. The spatial and temporal dynamics of the large neck actin granules and of actin fibres are also described.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-7-2035
1986-07-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/7/mic-132-7-2035.html?itemId=/content/journal/micro/10.1099/00221287-132-7-2035&mimeType=html&fmt=ahah

References

  1. Adams A. E. M., Pringle J. R. 1984; Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae. Journal of Cell Biology 98:934–945
    [Google Scholar]
  2. Anderson J. M., Soll D. R. 1984; Effects of zinc on stationary-phase phenotype and macromolecular synthesis accompanying outgrowth of Candida albicans. Infection and Immunity 46:13–21
    [Google Scholar]
  3. Bedell G., Soll D. R. 1979; The effects of low concentration of zinc on the growth and dimorphism of Candida albicans: evidence for zinc-resistant and zinc-sensitive pathways for mycelium formation. Infection and Immunity 26:348–354
    [Google Scholar]
  4. Bedell G., Werth A., Soll D. R. 1980; The regulation of nuclear migration and division during synchronous bud formation in released stationary phase cultures of the yeast Candida albicans. Experimental Cell Research 127:103–113
    [Google Scholar]
  5. Buffo J., Herman M., Soll D. R. 1984; A characterization of pH-regulated dimorphism inCandida albicans. Mycopathologia 85:21–30
    [Google Scholar]
  6. Herman M. A., Soll D. R. 1984; A comparison of volume growth during bud and mycelium formation in Candida albicans: a single cell analysis. Journal of General Microbiology 130:2219–2228
    [Google Scholar]
  7. Hoch H. C., Staples R. C. 1983; Visualization of actin in situ b rhodamine-conjugated phalloin in the fungus Uromyces phaseoli. European Journal of Cell Biology 32:52–58
    [Google Scholar]
  8. Kilmartin J. V., Adams A. E. M. 1984: Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces. Journal of Cell Biology 98:922–933
    [Google Scholar]
  9. Lee K. L., Buckley H. R., Campbell C. C. 1975; An amino acid liquid synthetic medium for development of mycelial and yeast forms of Candida albicans. Sabouraudia 13:148–153
    [Google Scholar]
  10. Mitchell L., Soll D. R. 1979A; Commitment to germ tube or bud formation during release from stationary phase in Candida albicans. Experimental Cell Research 120:167–179
    [Google Scholar]
  11. Mitchell L., Soll D. R. 1979 b; Temporal and spatial differences in septation during synchronous mycelium and bud formation by Candida albicans. Experimental Mycology 3:298–309
    [Google Scholar]
  12. Novick P., Bostein D. 1985; Phenotypic analysis of temperature-sensitive yeast actin mutants. Cell 40:405–416
    [Google Scholar]
  13. Soll D. R. 1984; The cell cycle and commitment to alternate cell fates in Candida albicans. In The Microbial Cell Cycle pp 143–162 Edited by Nurse P., Streiblova E. Boca Raton, Florida: CRC Press;
    [Google Scholar]
  14. Soll D.R. 1985a; The role of zinc in Candida dimorphism. In Current Topics in Medical Mycology vol 1 pp 258–285 Edited by McGinnis M.R. New York: Springer-Verlag;
    [Google Scholar]
  15. Soll D.R. 1985 b; Candida albicans. In Fungal Dimorphism with Emphasis on Fungi Pathogenic for Humans pp 167–195 Edited by Szaniszlo P. New York: Plenum Press;
    [Google Scholar]
  16. Soll D. R., Bedell G. 1978; Bud formation and the inducibility of pseudo-mycelium outgrowth during release from stationary phase in Candida albicans. Journal of General Microbiology 108:173–180
    [Google Scholar]
  17. Soll D. R., Herman M.A. 1983; Growth and the inducibility of mycelium formation in Candida albicans: a single-cell analysis using a perfusion chamber. Journal of GeneralMicrobiology 129:2809–2824
    [Google Scholar]
  18. Soll D. R., Stasi M., Bedell G. W. 1978; The regulation of nuclear migration and division during pseudo-mycelium outgrowth in the dimorphic yeast Candida albicans. Experimental Cell Research 116:207–215
    [Google Scholar]
  19. Soll D. R., Bedell G., Thiel J., Brummel M. 1981; The dependency of nuclear division on volume in the dimorphic yeast Candida albicans. Experimental Cell Research 133:55–62
    [Google Scholar]
  20. Soll D. R., Herman M. A., Staebell M. A. 1985; The involvement of cell wall expansion in the two modes of mycelium formation of Candida albicans. Journal of General Microbiology 131:2367–2375
    [Google Scholar]
  21. Staebell M., Soll D. R. 1985; Temporal and spatial differences in cell wall expansion during bud and mycelium formation in Candida albicans. Journal of General Microbiology 131:1467–1480
    [Google Scholar]
  22. Wulf E., Deboben A., Bautz F. A., Faustich H., Wieland TH. 1979; Fluorescent phallotoxin, a tool for the visualization of cellular actin. Proceedings of the National Academy of Sciences of the United States of America 76:4498–4502
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-7-2035
Loading
/content/journal/micro/10.1099/00221287-132-7-2035
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error