Numerical Analysis of Normalized Whole-cell Protein Profiles after Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis
Plikaytis, Brian D. and Carlone, George M. and Plikaytis, Bonnie B.,, 132, 2653-2660 (1986),
doi = https://doi.org/10.1099/00221287-132-9-2653,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= SUMMARY: A technique is described for mathematically normalizing whole-cell protein profiles after sodium dodecyl sulphate-polyacrylamide gel electrophoresis to obtain standardized absolute migration distances using two internal M r standards. A soft laser scanning densitometer was used to measure protein band migration distances in wet, silver-stained gels. The normalized values were superior to the unnormalized migration distances and common R F values in reducing the inter and intragel variability of the protein band positions. A procedure is described for clustering normalized bacterial protein profiles using a sample data set obtained from the type strains of four Legionella species.,
language=,
type=