%0 Journal Article %A Reed, William M. %A Dugan, Patrick R. %T Isolation and Characterization of the Facultative Methylotroph Mycobacterium ID-Y %D 1987 %J Microbiology, %V 133 %N 5 %P 1389-1395 %@ 1465-2080 %R https://doi.org/10.1099/00221287-133-5-1389 %I Microbiology Society, %X SUMMARY: A facultatively methylotrophic Mycobacterium was isolated from Cleveland Harbor, Ohio, USA. The isolate, designated ID-Y, used a wide range of carbon and energy sources including methane and several other hydrocarbons. It displayed a growth cycle from rod-shaped exponential-phase cells, with many cell pairs exhibiting V-formation, to cocco-bacillary stationary-phase cells. A fixation technique involving glutaraldehyde/alcian blue resulted in the observation of a three-layered cell wall. Isolate ID-Y has an ultrastructure similar to that of other mycobacteria, particularly Mycobacterium phlei and Mycobacterium flavum, which is presently classified as a Xanthobacter species. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-133-5-1389