Characterization of the Product of the Cloned fdhF Gene of Escherichia coli

SUMMARY: Random insertions of mini-MudII1734 lac gene fusion bacteriophage were constructed on plasmid pLW06, which carries the fdhF gene coding for benzyl-viologen-linked formate dehydrogenase. They allowed us to limit the size of the gene to a 3 kb fragment and to define its direction of transcription. The identification of the fdhF product as a 85 kDa protein was achieved after expression of derivative plasmids in a maxicell system.