1887

Abstract

The flagellar filaments of morphotype E isolates of characteristically possess an apparent helically arranged sheath structure, surrounding the central core of the filament. Reexamination of the type strains of H-serotypes belonging to morphotype E showed that all but serotype H34 possessed the expected morphology. Heterogeneity was observed in both the diameter of filaments from individual morphotype E strains and in the of individual flagellins. There was no apparent correlation between these two features. Monoclonal antibodies (MAbs) of the IgM class were raised against serotype H12 flagella. In Western immunoblotting and agglutination tests, the MAbs recognized the H12 antigen of six isolates with different 0:K antigen combinations. The MAbs were H-serotype-specific, with no significant reaction with the H-antigens of other morphotype E strains. The location of the serotype-specific H12 epitope(s) was studied by immunolabelling with colloidal gold markers. The epitope was surface-exposed and appeared to be helically arranged on the flagellar filament. The pattern of colloidal gold labelling was consistent with the possibility that the H12 serotype-specific epitope resides in the apparent sheath structure.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-7-1747
1988-07-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/7/mic-134-7-1747.html?itemId=/content/journal/micro/10.1099/00221287-134-7-1747&mimeType=html&fmt=ahah

References

  1. Burnette W. N. 1981; “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  2. Ewing W. H. 1986; The Genus Escherichia. In Edwards and Ewing’s Identification of Enterobacteriaceae, 4th edn. pp. 93–136 Ewing W. H. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  3. Hicks D., Molday R. S. 1986; Differential immunogold-dextran labeling of bovine and frog rod and cone cells using monoclonal antibodies against bovine rhodopsin. Experimental Eye Research 42:55–71
    [Google Scholar]
  4. Johnson R. C., Walsh M. P., Ely B., Shapiro L. 1979; Flagellar hook and basal complex of Caulobactercrescentus. Journal of Bacteriology 138:984–989
    [Google Scholar]
  5. Kuwajima G., Asaka J.-I., Fujiwara T., Fujiwara T., Node K., Kondo E. 1986; Nucleotide sequence of the hag gene encoding flagellin of Escherichia coli. Journal of Bacteriology 168:1479–1483
    [Google Scholar]
  6. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 221680–685
    [Google Scholar]
  7. Lam J. S., Lam M. Y. C., Macdonald L. A., Hancock R. E. W. 1987a; Visualisation of Pseudomonas aeruginosa O-antigens by using a protein A-dextran-colloidal gold conjugate with both immunoglobulin G and immunoglobulin Mmonoclonal antibodies. Journal of Bacteriology 169:3531–3538
    [Google Scholar]
  8. Lam J. S., Macdonald L. A., Lam M. Y. C., Duchesne L. G. M., Southam G. 1987b; Production and characterisation of monoclonal antibodies against serotype strains of Pseudomonas aeruginosa. Infection and Immunity 55:1051–1057
    [Google Scholar]
  9. Lawn A. M. 1977; Comparison of the flagellins from different flagellarmorphotypes of Escherichia coli. Journal of General Microbiology 101:121–130
    [Google Scholar]
  10. Lawn A. M., Ørskov I., Ørskov F. 1977; Morphological distinction between different H serotypes of Escherichia coli. Journal of General Microbiology 101:111–119
    [Google Scholar]
  11. Macnab R. M. 1987; Flagella. In Escherichia coli and Salmonella typhimurium.Cellular and Molecular Biology pp. 70–83 Neidhardt F. C., Ingraham J. L., Low K. B., Magasanik B., Schaecter M., Umbarger H. E. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  12. Ørskov F., Ørskov I. 1984; Serotyping of Escherichia coli. Methods in Microbiology 14:43–112
    [Google Scholar]
  13. Towbin M., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  14. Voller A., Bidwell D. E., Bartlett A. 1976; Enzyme immunoassays in diagnostic medicine. Bulletins of the World Health Organisation 53:55–65
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-7-1747
Loading
/content/journal/micro/10.1099/00221287-134-7-1747
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error