f An Immunoprotective Monoclonal Antibody Directed against Leptospira interrogans serovar copenhageni
- Authors: G. J. SCHOONE, C. O. R. EVERARD, H. KORVER, D. G. CARRINGTON, V. A. INNISS, J. BAULU, W. J. TERPSTRA
- Microbiology, January 1989 135: 73-78, doi: 10.1099/00221287-135-1-73
- Subject: Pathogenicity And Medical Microbiology
- Published Online:
A monoclonal antibody (mAb) was prepared by hybridoma technology in BALB/c mice immunized to Leptospira interrogans serovar copenhageni. This mAb agglutinated serovars copenhageni and icterohaemorrhagiae to high titres and protected hamsters, dogs and monkeys against challenge with a virulent strain of serovar copenhageni. The mAb gave protection to hamsters at dilutions up to 1 in 1000; at a 1 in 10 dilution the protective effect lasted for at least two weeks. Biochemical analysis by SDS-PAGE and Western blotting indicated that this mAb reacred with an epitope of a carbohydrate nature.
© Society for General Microbiology 1989 | Published by the Microbiology Society
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