f High Frequency Variation of Colony Morphology and Chromosome Reorganization in the Pathogenic Yeast Candida albicans
- Authors: TAKAHITO SUZUKI†, ISSEI KOBAYASHI, TOSHIO KANBE, KENJI TANAKA
- First Published Online: 01 February 1989, Microbiology 135: 425-434, doi: 10.1099/00221287-135-2-425
- Subject: Genetics And Molecular Biology
- Issue Published:
A clinical isolate of the pathogenic yeast Candida albicans varied in its colony morphology from smooth (o-smooth) to semi-rough type (SRT) and concomitantly lost its virulence for mice. In terms of DNA content, the smooth parent was near triploid when Saccharomyces cerevisiae strains of known ploidy were used as references. The SRT variant showed several features characteristic of polyploidy. From the SRT variant, revertant-like smooth (r-smooth) variants with recovered virulence were derived at a frequency of 5 × 10-3. The results of pulsed-field gel electrophoresis on chromosomal DNA showed changes in patterns of chromosome-sized DNA bands in the SRT variant as well as in r-smooth variants, which correlated with these variations. Correlations between colony morphology, state of ploidy and virulence of this asporogenous yeast are considered.
Present Address: Biological Laboratory, Faculty of Science, Nara Women's University, Nara 630, Japan.
© Society for General Microbiology 1989 | Published by the Microbiology Society
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