f Chlorate-resistant, Nitrate-utilizing (crn) Mutants of Fusarium moniliforme (Gibberella fujikuroi)
- Authors: Carla J. R. Klittich†, John F. Leslie
- First Published Online: 01 March 1989, Microbiology 135: 721-727, doi: 10.1099/00221287-135-3-721
- Subject: Genetics And Molecular Biology
- Issue Published:
SUMMARY: Five crn loci have been identified in Fusarium moniliforme. Mutants at these loci were chlorate resistant and able to utilize nitrate. The crnl locus was closely linked to, and may be allelic with, nit3, a regulatory gene controlling induction of the nitrate reductase pathway. The crn2 locus was linked to, but distinct from, the nit5 gene coding for a molybdenum-containing cofactor. The crn3 and crn4 loci were unlinked to any known gene affecting nitrate reduction. The crn5 locus was clearly linked to the nit1 gene coding for the nitrate reductase enzyme. Mutants of crn1 and crn5 had low but detectable levels of nitrate reductase activity; these mutations may be leaky alleles of nitrate reduction genes. crn2 mutants had normal nitrate reductase activity, and may be altered in nitrate uptake.
Present address: Ortho Research Center, Chevron Chemical Company, PO Box 4010, Richmond CA 94804-0010, USA.
© Society for General Microbiology, 1989 | Published by the Microbiology Society
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