1887

Abstract

Denitrification by and was studied using quadrupole membrane-inlet mass spectrometry to measure simultaneously and continuously dissolved gases. Evidence was provided for aerobic denitrification by both species: in the presence of O, NO production increased in , while that of N decreased; with , the concentrations of both N and NO increased on introducing O into the gas phase. Disappearance of NO was monitored in anaerobically and aerobically grown cells which were maintained either anaerobically or aerobically: the rate and extent of NO utilization by both species depended on growth and maintenance conditions. The initial rate of disappearance was most rapid under completely anaerobic conditions, and lowest rates occurred when cells were grown anaerobically and maintained aerobically. In nitrogen balance experiments both species converted over 87% of the added NO to N and NO under both anaerobic and aerobic maintenance conditions.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-9-2445
1989-09-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/9/mic-135-9-2445.html?itemId=/content/journal/micro/10.1099/00221287-135-9-2445&mimeType=html&fmt=ahah

References

  1. Alefounder P.R., Greenfield A.J., Mccarthy J., Ferguson S.J. 1983; Selection and organisation of denitrifying electron transfer pathways in Paracoccus denitrificans . Biochimica et biophysica acta 11A:20–39
    [Google Scholar]
  2. Bazylinski D.A., Blakemore R.P. 1983; Denitrification and assimilatory nitrate reductase in Aquaspirillum magnetotacticum . Applied and Environmental Microbiology 46:1118–1124
    [Google Scholar]
  3. Burnell J.N., John P., Whatley P.R. 1975; The reversibility of active sulphate transport in membrane vesicles of Paracoccus denitrificans . Biochemical Journal 150:527–536
    [Google Scholar]
  4. Degn H., Cox R.P., Lloyd D. 1985; Continuous measurement of dissolved gases in biochemical systems with the quadrupole mass spectrometer. In Methods of Biochemical Analysis pp. 165–194 Glick D. Edited by New York: Wiley-Interscience;
    [Google Scholar]
  5. DEPARTMENOFT OF ENVIRONMENT 1969 Analysis of Raw, Potable and Waste Water. London: HMSO;
    [Google Scholar]
  6. Hochstein L.I., Betlach M., Kritiko S.G. 1984; The effect of oxygen on denitrification during steady-state growth of Paracoccus halodenitri-ficans. . Archives of Microbiology 137:74–78
    [Google Scholar]
  7. John P. 1977; Aerobic and anaerobic bacterial respiration monitored by electrodes. Journal of General Microbiology 98:231–238
    [Google Scholar]
  8. Kaspar H.F. 1982; Nitrite reduction of nitrous oxide by propionibacteria: detoxication mechanism. Archives of Microbiology 133:126–130
    [Google Scholar]
  9. Koike I., Hattori A. 1975; Growth yield of a denitrifying bacterium, Pseudomonas denitrificans, under aerobic and anaerobic conditions. Journal of General Microbiology 88:1–10
    [Google Scholar]
  10. Krul J.M. 1976; Dissimilatory nitrate and nitrite reduction under aerobic conditions by an aerobically and anaerobically grown Alcaligenes sp. and by activated sludge. Journal of Applied Bacteriology 40:245–260
    [Google Scholar]
  11. Krul J.M., Veeningen R. 1977; The synthesis of dissimilatory nitrate reductase under aerobic conditions in a number of denitrifying bacteria isolated from activated sludge and drinking water. Water Research 11:39–43
    [Google Scholar]
  12. Lloyd D. 1985; Simultaneous dissolved O2 measurements in use of polarographic bioluminescence and mass spectrometric monitoring combined with dual wavelength spectrophotometry. In Gas Enzymology pp. 37–53 Degn H., Cox R.P., Toftlund H. Edited by Dordrecht: Reidel;
    [Google Scholar]
  13. Lloyd D., Scott R.I. 1983; Direct measurement of dissolved gases in microbial systems using membrane inlet mass spectrometry. Journal of Microbiological Methods 1:313–328
    [Google Scholar]
  14. Lloyd D., Scott R.I. 1985; Mass spectrometric monitoring of dissolved gases. In Microbial Gas Metabolism pp. 239–262 Poole R.K., Dow C.S. Edited by New York: Academic Press;
    [Google Scholar]
  15. Lloyd D., Boddy L., Davies K.J.P. 1987; Persistence of bacterial denitrification capacity under aerobic conditions: the rule rather than the exception. FEMS Microbiology Ecology 45185–190
    [Google Scholar]
  16. Meiburg J.B.M., Bruinenberg P.M., Harder W. 1980; Effect of dissolved oxygen tension on the metabolism of methylated amines in Hyphomicro-bium X in the presence and absence of aerobic denitrification. Journal of General Microbiology 120:453–463
    [Google Scholar]
  17. Nakajima M., Hayamizu T., Nishimura H. 1984; Inhibitory effect of oxygen on denitratification and denitrification in sludge from an oxidation ditch. Water Research 18:335–338
    [Google Scholar]
  18. Oren A., Blackburn T.H. 1979; Estimation of sediment denitrification rates at in situ nitrate concentrations. Applied and Environmental Microbiology 37:174–276
    [Google Scholar]
  19. Ottow J.C.G., Fabig W. 1985; Influence of oxygen aeration on denitrification and redox level in different batch cultures. In Planetry Ecology pp. 427–440 Caldwell D. E., Brierly J. A., Brierly C. L. Edited by New York: Van Nostrad Reinhold;
    [Google Scholar]
  20. Parsonage D., Greenfield A. JR Ferguson S.J. 1985; The high affinity of Paracoccus denitrificans cells for nitrate as an electron acceptor Analysis of possible mechanisms of nitrate and nitrite movement across the plasma membrane and the basis for inhibition by added nitrite of oxidase activity in permeabilized cells. Biochimica et biophy-sica acta 807:81–95
    [Google Scholar]
  21. Payne W.J. 1981 Denitrification. New York: Wiley-Interscience;
    [Google Scholar]
  22. Payne W.J. 1983; Bacterial denitrification: asset or defect. Bioscience 33:319–325
    [Google Scholar]
  23. Pichinoty F. 1973; La reduction bacterienne des composes oxygenes mineraux de l'azote. Bulletin de I'lnstitut Pasteur 71:317–395
    [Google Scholar]
  24. Robertson L.A., Kuenen J.G. 1983; Thiosphaera pantotropha gen. nov. sp. nov., a facultatively anaerobic, facultatively autotrophic sulphur bacterium. Journal of General Microbiology 129:2847–2855
    [Google Scholar]
  25. Robertson L.A., Kuenen J.G. 1984a; Aerobic denitrification-a controversy revived. Archives of Microbiology 139:351–354
    [Google Scholar]
  26. Robertson L.A., Kuenen J.G. 1984b; Aerobic denitrification: old wine in new bottles?. Antonie van Leeuwenhoek 50:525–544
    [Google Scholar]
  27. Thauer R.K., Jungermann K., Decker K. 1977; Energy conservation in chemotrophic anaerobic bacteria. Bacteriological Reviews 41:100–180
    [Google Scholar]
  28. Tiedje J.M., Sexstone A.J., Myrold D.D., Robinson J.A. 1982; Denitrification; ecological niches, competition and survival. Antonie uun Leeuwenhoek 48:569–583
    [Google Scholar]
  29. Voets J.P., Vanstaen H., Verstraete W. 1975; Removal of nitrogen from highly nitrogenous waste waters. Journal of the Water Pollution Control Federation 47:394–398
    [Google Scholar]
  30. Wilhelm E., Battion R., Wilcock R.J. 1977; Low pressure solubility of gases in liquid water. Chemical Reviews 77:219–230
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-9-2445
Loading
/content/journal/micro/10.1099/00221287-135-9-2445
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error