f A combined physical and genetic map of Pseudomonas aeruginosa PAO
- Authors: E. Ratnaningsih, S. Dharmsthiti, V. Krishnapillai, A. Morgan, M. Sinclair, B. W. Holloway
- Microbiology, December 1990 136: 2351-2357, doi: 10.1099/00221287-136-12-2351
- Subject: Genetics And Molecular Biology
- Published Online:
Summary: A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 Spel restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a Spel junction fragment library and fragments obtained by partial Spel digestion, both derived from the P. aeruginosa PAO chromosome.
© Society for General Microbiology 1990 | Published by the Microbiology Society
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