@article{mbs:/content/journal/micro/10.1099/00221287-136-12-2351, author = "Ratnaningsih, E. and Dharmsthiti, S. and Krishnapillai, V. and Morgan, A. and Sinclair, M. and Holloway, B. W.", title = "A combined physical and genetic map of Pseudomonas aeruginosa PAO", journal= "Microbiology", year = "1990", volume = "136", number = "12", pages = "2351-2357", doi = "https://doi.org/10.1099/00221287-136-12-2351", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-136-12-2351", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library and fragments obtained by partial SpeI digestion, both derived from the P. aeruginosa PAO chromosome.", }