f Determination of turgor pressure in Bacillus subtilis: a possible role for K+ in turgor regulation
- Authors: Adrian M. Whatmore†, Robert H. Reed‡
- Microbiology, December 1990 136: 2521-2526, doi: 10.1099/00221287-136-12-2521
- Subject: Physiology And Growth
- Published Online:
Summary: The effects of hypersaline treatment (osmotic upshock) on cell water relations were examined in the Gram-positive bacterium Bacillus subtilis using particle size analysis. Application of the Boyle-van't Hoflf relationship (cell volume versus reciprocal of external osmolality) permitted direct determination of turgor pressure, which was approximately 0·75 osmol kg−1 (1·9 MPa) in exponentially growing bacteria in a defined medium. The abolition of turgor pressure immediately after upshock and the subsequent recovery of turgor were investigated. Recovery of turgor was K+ dependent. Calculation of turgor by an alternative method involving spectrophotometric analysis of shrinkage gave somewhat lower estimates of turgor pressure.
Present address: Department of Microbiology, New Medical School, University of Newcastle, Newcastle upon Tyne NE2 4HH, UK.
Present address: Department of Chemical and Life Sciences, Newcastle Polytechnic, Newcastle upon Tyne NE1 8ST, UK.
© Society for General Microbiology 1990 | Published by the Microbiology Society
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