Determination of turgor pressure in Bacillus subtilis: a possible role for K+ in turgor regulation
Whatmore, Adrian M. and Reed, Robert H.,, 136, 2521-2526 (1990),
doi = https://doi.org/10.1099/00221287-136-12-2521,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= The effects of hypersaline treatment (osmotic upshock) on cell water relations were examined in the Gram-positive bacterium Bacillus subtilis using particle size analysis. Application of the Boyle-van't Hoflf relationship (cell volume versus reciprocal of external osmolality) permitted direct determination of turgor pressure, which was approximately 0·75 osmol kg−1 (1·9 MPa) in exponentially growing bacteria in a defined medium. The abolition of turgor pressure immediately after upshock and the subsequent recovery of turgor were investigated. Recovery of turgor was K+ dependent. Calculation of turgor by an alternative method involving spectrophotometric analysis of shrinkage gave somewhat lower estimates of turgor pressure.,
language=,
type=