@article{mbs:/content/journal/micro/10.1099/00221287-136-2-331, author = "Hamilton, A. J. and Bartholomew, M. A. and Fenelon, L. E. and Figueroa, J. and Hay, R. J.", title = "A murine monoclonal antibody exhibiting high species specificity for Histoplasma capsulatum var. capsulatum", journal= "Microbiology", year = "1990", volume = "136", number = "2", pages = "331-335", doi = "https://doi.org/10.1099/00221287-136-2-331", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-136-2-331", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "A monoclonal antibody (mAb) exhibiting a high degree of species specificity for the yeast phase of the dimorphic fungus Histoplasma capsulatum was produced by a modification of the standard mAb production protocol. The technique for generating mAbs involved the use of the immunosuppressive drug cyclophosphamide to diminish the response in mice to immunodominant cross-reactive epitopes. This mAb exhibited clear specificity and did not react by ELISA with the closely related genera Blastomyces, Paracoccidioides and Sporothrix. In Western blots it recognized a linear determinant on a 70–75 kDa molecule in H. capsulatum antigen, with an extremely faint reactivity to antigens of identical molecular mass derived from Sporothrix and Paracoccidoides, and no reactivity against Blastomyces antigen.", }