%0 Journal Article %A Hamilton, A. J. %A Bartholomew, M. A. %A Fenelon, L. E. %A Figueroa, J. %A Hay, R. J. %T A murine monoclonal antibody exhibiting high species specificity for Histoplasma capsulatum var. capsulatum %D 1990 %J Microbiology, %V 136 %N 2 %P 331-335 %@ 1465-2080 %R https://doi.org/10.1099/00221287-136-2-331 %I Microbiology Society, %X A monoclonal antibody (mAb) exhibiting a high degree of species specificity for the yeast phase of the dimorphic fungus Histoplasma capsulatum was produced by a modification of the standard mAb production protocol. The technique for generating mAbs involved the use of the immunosuppressive drug cyclophosphamide to diminish the response in mice to immunodominant cross-reactive epitopes. This mAb exhibited clear specificity and did not react by ELISA with the closely related genera Blastomyces, Paracoccidioides and Sporothrix. In Western blots it recognized a linear determinant on a 70–75 kDa molecule in H. capsulatum antigen, with an extremely faint reactivity to antigens of identical molecular mass derived from Sporothrix and Paracoccidoides, and no reactivity against Blastomyces antigen. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-136-2-331