RT Journal Article SR Electronic(1) A1 Cooper, Morris D. A1 Rapp, Judy A1 Jeffery-Wiseman, Christine A1 Barnes, Robert C. A1 Stephens, David S.YR 1990 T1 Chlamydia trachomatis infection of human fallopian tube organ cultures JF Microbiology, VO 136 IS 6 SP 1109 OP 1115 DO https://doi.org/10.1099/00221287-136-6-1109 PB Microbiology Society, SN 1465-2080, AB The pathogenic events that precede Chlamydia trachomatis salpingitis in the human fallopian tube have not been fully described. We used a model of human fallopian tubes in organ culture (HFTOC) infected with strain E/UW-5/CX of C. trachomatis to study these events. The model supported sustained C. trachomatis infection as demonstrated by recovery of viable C. trachomatis from medium and tissue over 5–7 d. However, the level of infectivity was low. Maximal infection occurred at 72 h after initial inoculation. In contrast to gonococcal infection of the HFTOC, C. trachomatis did not damage overall ciliary function of HFTOC. However, a local direct cytotoxic effect characterized by loss of microvilli and disruption of cell junctions was noted when multiple chlamydial elementary bodies attached to mucosal cells. Beginning at 24 h, and continuing throughout the course of C. trachomatis infection of HFTOC, ruptured epithelial cells releasing elementary bodies were noted. Chlamydial inclusions were seen in the mucosa by 72 h in ~6% of both ciliated and nonciliated epithelial cells. Mucosal inclusions contained all forms of the C. trachomatis developmental cycle. These data suggest that factors present in the human fallopian tube may limit susceptibility to chlamydial infection but support the use of the HFTOC model in the study of the pathogenesis of C. trachomatis salpingitis., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-136-6-1109