1887

Abstract

A detailed restriction map of the virulence plasmid of has been determined and used for comparison with the virulence plasmid from Two regions were identified which appeared to be similar based on blotting and restriction data. One, of about 22 kb, encompassed the virulence region; the other, of about 8 kb, was outside it. The locations of 259 transposon insertions on the plasmid were determined and related to their effect on virulence. One gene involved in virulence but outside the essential virulence region was shown to affect citrate metabolism.

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1990-06-01
2024-04-18
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References

  1. Baird G.D., Manning E.J. 1984; Molecular evidence for plasmid involvement in the virulence of Salmonella dublin. In Priority Aspects of Salmonellosis Research pp 147–153 Larsen H. E. Edited by Brussels: Commission of the European Communities; Publication EUR 9197EN
    [Google Scholar]
  2. Baird G.D., Manning E.J., Jones P.W. 1985; Evidence for related virulence sequences in plasmids of Salmonella dublin and Salmonella typhimurium. Journal of General Microbiology 131:1815–1823
    [Google Scholar]
  3. Barrow P.A., Lovell M.A. 1988; The association between a large molecular mass plasmid and virulence in a strain of Salmonella pullorum. Journal of General Microbiology 134:2307–2316
    [Google Scholar]
  4. Barrow P.A., Simpson J.M., Lovell M.A., Binns M.M. 1987; Contribution of Salmonella gallinarum large plasmid toward virulence in fowl typhoid. Infection and Immunity 55:388–392
    [Google Scholar]
  5. Beninger P.R., Chikami G., Tanabe K., Roudier C., Fierer J., Guiney D.G. 1988; Physical and genetic mapping of the Salmonella dublin virulence plasmid pSDL2. Journal of Clinical Investigation 81:1341–1347
    [Google Scholar]
  6. Bennett P.M., Grinsted J., Richmond M.H. 1977; Transposition of Tn A does not generate deletions. Molecular and General Genetics 154:205–212
    [Google Scholar]
  7. Davis R.W., Botstein D., Roth J.R. 1980 Advanced Bacterial Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  8. Gulig P.A., Curtiss R.III 1988; Cloning and transposon insertion mutagenesis of virulence genes of the 100-kilobase plasmid of Salmonella typhimurium. Infection and Immunity 56:3262–3271
    [Google Scholar]
  9. Heffron F., Rubens C., Falkow S. 1975; Translocation of a plasmid DNA sequence which mediates ampicillin resistance: molecular nature and specificity of insertion. Proceedings of the National Academy of Sciences of the United States of America 72:3623–3627
    [Google Scholar]
  10. Helmuth R., Stephen R., Bunge C., Hoog B., Steinbeck A., Bulling E. 1985; Epidemiology of virulence-associated plasmids and outer membrane protein patterns within seven common Salmonella serotypes. Infection and Immunity 48:175–182
    [Google Scholar]
  11. Ish-Horowitz D., Burke J.F. 1981; Rapid and efficient cosmid cloning. Nucleic Acids Research 9:2989–2998
    [Google Scholar]
  12. Jones G.W., Rabert D.K., Svinarich D.M., Whitfield H.J. 1982; Association of adhesive, invasive and virulent phenotypes of Salmonella typhimurium with autonomous 60-megadalton plasmids. Infection and Immunity 38:476–486
    [Google Scholar]
  13. Jones P.W., Collins P., Lax A. 1988; The role of large plasmids in the pathogenesis of salmonellosis in cattle. Journal of Medical Microbiology 27:x
    [Google Scholar]
  14. Kawahara K., Haraguchi Y., Tsuchimoto M., Terakado N., Danbara H. 1988; Evidence of correlation between 50-kilobase plasmid of Salmonella choleraesuis and its virulence. Microbial Pathogenesis 4:155–163
    [Google Scholar]
  15. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning, a Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Manning E.J., Baird G.D., Jones P.W. 1986; The role of plasmid genes in the pathogenicity of Salmonella dublin. Journal of Medical Microbiology 21:239–243
    [Google Scholar]
  17. Mcclelland M., Jones R., Patel Y., Nelson M. 1987; Restriction endonucleases for pulse field mapping of bacterial genomes. Nucleic Acids Research 15:5985–6005
    [Google Scholar]
  18. Michiels T., Popoff M.Y., Durviaux S., Coynault C., Cornelis G. 1987; A new method for the physical and genetic mapping of large plasmids: application to the localisation of the virulence determinants on the 90 kbp plasmid of Salmonella typhimurium. Microbial Pathogenesis 3:109–116
    [Google Scholar]
  19. Nakamura M., Sato S., Ohya T., Suzuki S., Ikeda S. 1985; Possible relationship of a 36-megadalton Salmonella enteritidis plasmid to virulence in mice. Infection and Immunity 47:831–833
    [Google Scholar]
  20. Norel F., Coynault C., Miras I., Hermant D., Popoff M. Y. 1989a; Cloning and expression of plasmid DNA sequences involved in Salmonella serotype typhimurium virulence. Molecular Microbiology 3:733–743
    [Google Scholar]
  21. Norel F., Pisano M.-R., Nicoli J., Popoff M.Y. 1989b; Nucleotide sequence of the plasmid-borne virulence gene mkfA encoding a 28 kDa polypeptide from Salmonella typhimurium. Research in Microbiology 140:263–265
    [Google Scholar]
  22. Pardon P., Popoff M.Y., Coynault C., Marly J., Miras I. 1986; Virulence-associated plasmids of Salmonella serotype typhimurium in experimental murine infection. Annales de I'Institut Pasteur/Microbiologie 137B:47–60
    [Google Scholar]
  23. Popoff M.Y., Miras I., Coynault C., Lasselin C., Pardon P. 1984; Molecular relationships between virulence plasmids of Salmonella serotypes typhimurium and dublin and large plasmids of other Salmonella serotypes. Annales de l’Institut Pasteur/Microbiologie 135A:389–398
    [Google Scholar]
  24. Pullinger G.D., Baird G.D., Williamson C.M., Lax A.J. 1989; Nucleotide sequence of a plasmid gene involved in the virulence of salmonellas. Nucleic Acids Research 17:79–83
    [Google Scholar]
  25. Rhen M., Virtanen M., Makela P.H. 1989; Localisation by insertional mutagenesis of a virulence-associated region on the Salmonella typhimurium 96 kilobase pair plasmid. Microbial Pathogenesis 6:153–158
    [Google Scholar]
  26. Rose R.E. 1988; The nucleotide sequence of pACYC184. Nucleic Acids Research 16:355
    [Google Scholar]
  27. Taira S., Rhen M. 1989; Identification and genetic analysis of mkaA-a gene of the Salmonella typhimurium virulence plasmid necessary for intracellular growth. Microbial Pathogenesis 7:165–173
    [Google Scholar]
  28. Terakado N., Sekizaki T., Hashimoto K., Naitoh S. 1983; Correlation between the presence of a fifty-megadalton plasmid in Salmonella dublin and virulence for mice. Infection and Immunity 41:443–444
    [Google Scholar]
  29. Tu C.P., Cohen S.N. 1980; Translocation specificity of the TnJ element: characterization of sites of multiple insertions. Cell 19:151–160
    [Google Scholar]
  30. Williamson C.M. 1988 Plasmid involvement in the virulence of Salmonella bacteria PhD thesis University of Reading;
    [Google Scholar]
  31. Williamson C.M., Baird G.D., Manning E.J. 1988a; A common virulence region on plasmids from eleven serotypes of Salmonella. Journal of General Microbiology 134:975–982
    [Google Scholar]
  32. Williamson C.M., Pullinger G.D., Lax A.J. 1988b; Identification of an essential virulence region on Salmonella plasmids. Microbial Pathogenesis 5:469–473
    [Google Scholar]
  33. Woodward M.J., Mclaren I., Wray C. 1989; Distribution of virulence plasmids within salmonellae. Journal of General Microbiology 135:503–511
    [Google Scholar]
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