1887

Abstract

Summary: The oral micro-organism ATCC 25975 synthesizes extracellular glucosyltransferases (GTFs) which polymerize the glucose moiety of sucrose into glucan polymers. Two separate genes encoding the activities of a GTF-I (a GTF that synthesizes an insoluble product) and a GTF-S (a GTF that synthesizes soluble product) were cloned into bacteriophage L47.1. The inserts in the -clones were characterized by restriction mapping and Southern hybridization and were found to overlap, implying that the two genes lay very close to one another on the chromosome. Both genes were subcloned into phagemid vector pIBI30 where they were expressed at a high level. The GTF-I-encoding gene was named and the GTF-S-encoding gene, . Nucleotide sequencing showed that and most probably were closely related to the genes of the mutans streptococci. Sequence alignment also indicated that lay very close to and downstream from , and that both were transcribed in the same direction.

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1991-11-01
2024-04-16
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References

  1. Aduse-Opoku J., Gilpin M. L., Russell R. R. B. 1989; Genetic and antigenic comparison of Streptococcus mutatis fructosyl-transferase and glucan-binding protein. FEMS Microbiology Letters 50:279–282
    [Google Scholar]
  2. Coykendall A. L. 1989; Classification and identification of the viridans streptococci. Clinical Microbiology Reviews 2:315–328
    [Google Scholar]
  3. Eifuku H., Yoshimitsu-Narita A., Sato S., Yakushiji T., Inoue M. 1989; Production and partial characterization of the extracellular polysaccharides from oral Streptococcus salivarius . Carbohydrate Research 194:247–260
    [Google Scholar]
  4. Dretzen G., Bellard M., Sassone-Corsi P., Chambon P. 1981; A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Analytical Biochemistry 112:295–298
    [Google Scholar]
  5. Drucker D. B., Shakespeare A. P., Green R. M. 1984; The production of dental plaque and caries by the bacterium Streptococcus salivarius in gnotobiotic WAG/RIJ rats. Archives of Oral Biology 29:437–443
    [Google Scholar]
  6. Ferretti J. J., Gilpin M. L., Russell R. R. B. 1987; Nucleotide sequence of a glucosyltransferase gene from Streptococcus sobrinus MFe28. Journal of Bacteriology 169:4271–4278
    [Google Scholar]
  7. Gibbons R. J., van Houte J. 1978; Oral bacterial ecology. In Textbook of Oral Biology684–705 Shaw J. H., Sweeney E. A., Cappuccino C. C., Mellor S. M. Philadelphia: W. B. Saunders;
    [Google Scholar]
  8. Gilmore K. S., Russell R. R. B., Ferretti J. J. 1990; Analysis of the Streptococcus downei gtfS gene, which specifies a glucosyltransferase that synthesizes soluble glucans. Infection and Immunity 58:2452–2458
    [Google Scholar]
  9. Gough J. A., Murray N. E. 1983; Sequence diversity among related genes for recognition of specific targets in DNA molecules. Journal of Molecular Biology 166:1–19
    [Google Scholar]
  10. Hamilton I. R. 1967; Synthesis and degradation of intracellular polyglucose in Streptococcus salivarius. Canadian Journal of Microbiology 14:65–77
    [Google Scholar]
  11. von Heune G. 1990; The signal peptide. Journal of Membrane Biology 115:195–201
    [Google Scholar]
  12. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  13. Honda O., Kato C., Kuramitsu H. K. 1990; Nucleotide sequence of the Streptococcus mutans gtfD gene encoding the glucosyltransferase-S enzyme. Journal of General Microbiology 136:2099–2105
    [Google Scholar]
  14. Krasse B., Carlsson J. 1970; Various types of streptococci and experimental caries in hamsters. Archives of Oral Biology 15:25–32
    [Google Scholar]
  15. Loenen W. A., Brammar W. J. 1980; A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes. Gene 10:249–259
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Markevics L. J., Jacques N. A. 1985; Enhanced secretion of glucosyltransferase by changes in potassium ion concentrations is accompanied by an altered pattern of membrane fatty acids in Streptococcus salivarius. Journal of Bacteriology 161:989–994
    [Google Scholar]
  18. Murray N. E., Brammar W. J., Murray K. 1977; Lambdoid phages that simplify the recovery of in vitro recombinants. Molecular and General Genetics 150:53–61
    [Google Scholar]
  19. Pearson W. R. 1990; Rapid and sensitive sequence comparison with FastA and FastP. Methods in Enzymology 188:63–98
    [Google Scholar]
  20. Perry D., Kuramitsu H. K. 1990; Linkage of sucrose-metabolizing genes in Streptococcus mutans. Infection and Immunity 58:3462–3464
    [Google Scholar]
  21. Pitty (nee Markevics) L. J., Jacques N. A. 1987; The influence of incorporation of octadecenoic acid on the cell-associated fructosyltransferase and the extracellular glucosyltransferase activities of Streptococcus salivarius. Journal of General Microbiology 133:3565–3573
    [Google Scholar]
  22. Pitty L. J., Giffard P. M., Gilpin M. L., Russell R. R. B., Jacques N. A. 1989; Cloning and expression of glycosyltransferase activities from Streptococcus salivarius. Journal of Dental Research 68:1681–1682
    [Google Scholar]
  23. Rölla G., Ciardi J. E., Eggen K. H., Bowen W. F., Afseth J. 1983; Free glucosyl- and fructosyl-transferase in human saliva and adsorption of these enzymes to teeth in vivo. In Proceedings: Glucosyltransferase, Glucans, Sucrose and Dental Caries. Special Supplement to Chemical Senses21–29 Doyle J. R., Ciardi J. E. Oxford: IRL Press;
    [Google Scholar]
  24. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 745463–5467
    [Google Scholar]
  25. Schleifer K. H., Kilpper-Balz R. 1987; Molecular and chemotaxonomic approaches to the classification of streptococci, enterococci and lactococci: a review. Systematic and Applied Microbiology 10:1–19
    [Google Scholar]
  26. Shiroza T., Ueda S., Kuramitsu H. K. 1987; Sequence analysis of the gtfB gene from Streptococcus mutans. Journal of Bacteriology 169:4263–4270
    [Google Scholar]
  27. Silhavy T. J., Berman M. L., Enquist L. W. 1984 Experiments with Gene Fusions Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Smith D. J., Taubman M. A. 1978; Immunology and dental caries. In Textbook of Oral Biology992–1006 Shaw J. H., Sweeney E. A., Cappuccino C. C., Meller S. M. Philadelphia: W. B. Saunders;
    [Google Scholar]
  29. Ueda S., Shiroza T., Kuramitsu H. K. 1988; Sequence analysis of the gtfC gene from Streptococcus mutans GS-5. Gene 69:101–109
    [Google Scholar]
  30. Walker G. J., Jacques N. A. 1987; Polysaccharides of oral streptococci. In Sugar Transport and Metabolism in Gram-positive Bacteria39–68 Reizer J., and Peterkofsky A., Chichester: Ellis Horwood;
    [Google Scholar]
  31. Wong C., Hefta S. A., Paxton R. J., Shively J. E., Mooser G. 1990; Size and subdomain architecture of the glucan-binding domain of the sucrose: 3-α-D-glucosyltransferase from Streptococcus sobrinus. Infection and Immunity 58:2165–2170
    [Google Scholar]
  32. Yannisch-Perron C., Vieira J., Messing J. 1985; Improved M13 cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
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