1887

Abstract

Summary

General properties of S-adenosylmethionine decarboxylase (SAMDC) from were studied. Dormant spores of the fungus did not contain detectable levels of the enzyme, but it started to be synthesized at early stages of spore germination. Kinetics of synthesis changed before emergence of the germ tube, with a corresponding increase in a second SAMDC activity which, in contrast to the one originally synthesized, was not activated by putrescine. Development of the second enzyme activity required protein synthesis. Neither enzymic activity was stimulated by Mg. Addition of the SAMDC inhibitor methylglyoxal bis-(guanylhydrazone) (MGBG) stopped fungal development in growth phase Ia: cells became spherical and showed ultrastructural alterations. Although MGBG inhibited polyamine formation, it barely inhibited protein and RNA biosynthesis during the first hour of incubation. However, at later periods, biosynthesis of both macromolecules was strongly decreased. When MGBG was added to growth media 3 h after inoculation of spores, it did not affect spore germination and outgrowth. A hypothesis for two different roles of spermidine and putrescine in spore germination is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-2-307
1991-02-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/2/mic-137-2-307.html?itemId=/content/journal/micro/10.1099/00221287-137-2-307&mimeType=html&fmt=ahah

References

  1. Bartnicki-Garcia S., Lippman E. 1977; Polarization of cell wall synthesis during spore germination. Experimental Mycology 1:230–240
    [Google Scholar]
  2. Bartnicki-Garcia S., Nickerson W. J. 1962; Nutrition, growth and morphogenesis of Mucor rouxii . Journal of Bacteriology 84:841–858
    [Google Scholar]
  3. Calvo-Mendez C., Martinez-Pacheco M., Ruiz-Herrera J. 1987; Regulation of ornithine decarboxylase activity in Mucor bacilliformis and Mucor rouxii . Experimental Mycology 11:270–277
    [Google Scholar]
  4. Cano C., Ruiz-Herrera J. 1988; Developmental stages during the germination of Mucor sporangiospores. Experimental Mycology 12:47–59
    [Google Scholar]
  5. Cano C, Herrera-Estrella L., Ruiz-Herrera J. 1988; DNA methylation and polyamines in regulation of development of the fungus Mucor rouxii . Journal of Bacteriology 170:5946–5948
    [Google Scholar]
  6. Guilbault G. G., Brignac P., Zimmer M. 1968; Homovanillic acid as a fluorometric substrate for oxidative enzymes. Analytical applications of the peroxidase, glucose oxidase, and xanthine oxidase systems. Analytical Chemistry 40:190–196
    [Google Scholar]
  7. Gupta S., Srivastava D. K., Shukla O. P. 1986; Inhibition of polyamine metabolism induces encystation in Acanthamoeba culbert-soni . Current Science 55:732–734
    [Google Scholar]
  8. Gupta S., Shukla O. P., Walter R. D. 1987; Putrescineactivated 5-adenosylmethionine decarboxylase from Acanthamoeba culbertsoni . Molecular and Biochemical Parasitology 23:247–252
    [Google Scholar]
  9. Heby O. 1981; Role of polyamines in the control of cell proliferation and differentiation. Differentiation 19:1–20
    [Google Scholar]
  10. Kaul S. M., Imam S. A., Shukla O. P. 1985; Growth inhibition of E. histolytica by methylglyoxal bis-(guanylhydrazone). Current Science 54:800–807
    [Google Scholar]
  11. Nickerson K. W., Dunkle L. D., van Etten J. L. 1977; Absence of spermine in filamentous fungi. Journal of Bacteriology 129:173–176
    [Google Scholar]
  12. Oka T., Perry J. W. 1974; Spermidine as possible mediator of glucocorticoid effect on milk protein synthesis in mouse mammary epithelium in vitro . Journal of Biological Chemistry 249:7647–7652
    [Google Scholar]
  13. Pegg A. E. 1984; S-Adenosylmethionine decarboxylase: a brief review. Cell Biochemical Function 2:11–15
    [Google Scholar]
  14. Pegg A. E. 1986; Recent advances in the biochemistry of polyamines in eukaryotes. Biochemical Journal 234:249–262
    [Google Scholar]
  15. Pegg A. E., McCann P. P. 1982; Polyamine metabolism and function. American Journal of Physiology 243 (Cell Physiology 12)C212–C221
    [Google Scholar]
  16. Ruiz-Herrera J., Calvo-Mendez C. 1987; Effect of ornithine decarboxylase inhibitors on the germination of sporangiospores of Mucorales. Experimental Mycology 11:287–296
    [Google Scholar]
  17. Ruiz-Herrera J., Valenzuela C, Martinez-Cadena G., Obregon A. 1989; Alterations in the vesicular pattern and wall growth of Phycomyces induced by the calcium ionophore A231 87. Protoplasma 148:15–25
    [Google Scholar]
  18. Stevens L., Winther M. D. 1979; Spermine, spermidine and putrescine in fungal development. Advances in Microbial Physiology 19:63–148
    [Google Scholar]
  19. Suresh M. R., Adiga P. R. 1977; Putrescine-sensitive (artifactual) and insensitive (biosynthetic) S-adenosyl-L-methionine decarboxylase activities of Lathyrus sativus seedling. European Journal of Biochemistry 79:511–518
    [Google Scholar]
  20. Tabor C. W., Tabor H. 1984; Polyamines. Annual Review of Biochemistry 55:749–790
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-2-307
Loading
/content/journal/micro/10.1099/00221287-137-2-307
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error