RT Journal Article SR Electronic(1) A1 Fukunaga, Masahito A1 Yanagihara, Yasutake A1 Sohnaka, MasakoYR 1992 T1 The 23S/5S ribosomal RNA genes (rrl/rrf) are separate from the 16S ribosomal RNA gene (rrs) in Borrelia burgdorferi, the aetiological agent of Lyme disease JF Microbiology, VO 138 IS 5 SP 871 OP 877 DO https://doi.org/10.1099/00221287-138-5-871 PB Microbiology Society, SN 1465-2080, AB SUMMARY: DNA fragments containing the rRNA genes for Borrelia burgdorferi strain B31 were cloned in bacteriophage λ EMBL3. A restriction map of the fragments was constructed and the organization of the rRNA genes was determined by Southern hybridization. One genomic DNA fragment contained a single copy of the rrs sequence and another cloned fragment contained both rrl and rrf sequences. The results revealed that the rrs gene is located separately from the set of rrl/rrf genes, suggesting that these rRNA genes are expressed independently in B. burgdorferi , UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-138-5-871