f Exogenous siderophore-mediated iron uptake in Pseudomonas aeruginosa: possible involvement of porin OprF in iron translocation
- By Jean-Marie Meyer*
- *Author for correspondence. Tel. 88 41 61 53; fax 88 60 75 50; email MBIO@FRIBCP51.EARN/BITNET.
- First Published Online: 01 May 1992, Microbiology 138: 951-958, doi: 10.1099/00221287-138-5-951
- Subject: Physiology And Growth
- Issue Published:
SUMMARY: In addition to the two siderophores pyoverdine and pyochelin synthesized by Pseudomonas aeruginosa ATCC 15692 (strain PAO1), several siderophores produced by other bacteria or fungi, namely cepabactin, salicylic acid, desferriferrichrysin, desferriferricrocin, desferriferrioxamine B, desferriferrioxamine E and coprogen, were able to promote iron uptake with variable efficiencies into this bacterium. For most of these siderophores, these results were consistent with the growth stimulation produced by the same compounds in a plate bioassay. Desferriferrichrome A, enterobactin and desferriferrirubin, however, did not promote iron uptake, although enterobactin and desferriferrirubin stimulated bacterial growth. These paradoxical data are discussed in view of siderophore-inducible iron uptake systems, as demonstrated recently for enterobactin. Among the strains tested, including the wild-type PAO1, the pyoverdine-less mutant PAO6606 and the two porin-mutants P. aeruginosa H636 (oprF::Ω) and P. aeruginosa H673 (oprD::Tn501), only for the porin-OprF mutant were fewer siderophores able to promote iron uptake compared to the other strains. Such results suggest that beside specific routes for iron uptake P. aeruginosa is also able to take up siderophore-liganded iron through OprF.
© Society for General Microbiology 1992 | Published by the Microbiology Society
Article metrics loading...
Full text loading...
Author and Article Information
/content/journal/micro/10.1099/00221287-138-5-951dcterms_title,dcterms_subject,pub_serialTitlepub_serialIdent:journal/micro AND -contentType:BlogPost104
/content/journal/micro/10.1099/00221287-138-5-951dcterms_title,dcterms_subject-pub_serialIdent:journal/micro AND -contentType:BlogPost104
Figure data loading....