f A survey of Col plasmids in natural isolates of Escherichia coli and an investigation into the stability of Col-plasmid lineages
- Authors: MARGARET A. RILEY*, DAVID M. GORDON
- *Author for correspondence. Present address: Department of Biology, Osborn Memorial Laboratories, Yale University, New Haven, CT 06511, USA. Tel. (203) 432 3875; fax (203) 432 3854.
- First Published Online: 01 July 1992, Microbiology 138: 1345-1352, doi: 10.1099/00221287-138-7-1345
- Subject: Genetics And Molecular Biology
- Issue Published:
A survey of colicins in the ECOR reference collection of Escherichia coli is presented. Twenty-five of the 72 ECOR strains exhibited a phenotype consistent with colicin production and E. coli isolated from human hosts were more likely to be colicinogenic than those from animal hosts. Multiple representatives of two Col plasmids, lowmolecular-mass ColE1 plasmids and high-molecular-mass, conjugative ColIa plasmids were isolated from the ECOR collection and were examined with a combination of restriction fragment and Southern analysis. These data suggested that ColE1 plasmids comprise a stable (cohesive) plasmid lineage, while ColIa plasmids represent a family of distinct plasmid lineages united by the presence of the colicin Ia operon.
© Society for General Microbiology, 1992 | Published by the Microbiology Society
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/content/journal/micro/10.1099/00221287-138-7-1345dcterms_title,dcterms_subject-pub_serialIdent:journal/micro AND -contentType:BlogPost104
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