f Adhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cells
- Authors: GILLES CHAUVIÈRE, MARIE-HÉLÈNE COCONNIER, SOPHIE KERNÉIS, JACKY FOURNIAT, ALAIN L. SERVIN*
- *Author for correspondence. Fax 1 46 83 13 03.
- Microbiology, August 1992 138: 1689-1696, doi: 10.1099/00221287-138-8-1689
- Subject: Pathogenicity And Medical Microbiology
- Published Online:
Twenty-five strains of lactobacilli were tested for their ability to adhere to human enterocyte-like Caco-2 cells in culture. Seven Lactobacillus strains adhered well to the Caco-2 cells, of which three possessed calcium-independent adhesion properties. A high level of calcium-independent adhesion was observed with the human stool isolate Lactobacillus acidophilus strain LB. Scanning electron microscopy revealed that this strain adhered to the apical brush border of the cells. Adhesion increased in parallel with the morphological and functional differentiation of the Caco-2 cells. Two Lactobacillus components were involved in this adhesion. One was protease-resistant and bacterial-surface-associated; the other was heat-stable, extracellular and protease-sensitive.
© Society for General Microbiology, 1992 | Published by the Microbiology Society
Article metrics loading...
Full text loading...
Author and Article Information
/content/journal/micro/10.1099/00221287-138-8-1689dcterms_title,dcterms_subject,pub_serialTitlepub_serialIdent:journal/micro AND -contentType:BlogPost64
/content/journal/micro/10.1099/00221287-138-8-1689dcterms_title,dcterms_subject-pub_serialIdent:journal/micro AND -contentType:BlogPost64
Figure data loading....