1887

Abstract

SUMMARY: A brief exposure ( 20 min) of the yeast to the phytotoxin syringomycin was sufficient to kill the cell. The protective effect of sterols against this cytotoxicity of syringomycin was investigated. Syringomycin was much more toxic to growing cells than to stationary-phase cells. The cytotoxicity of syringomycin was reduced in an environment containing sterols. Cytotoxicity of syringomycin at 3 fig ml ( 2.5 μm) was completely abolished by the simultaneous presence of 10 μM-cholesterol in the medium. Cholesterol acetate had no protective effect. Ergosterol, sitosterol and stigmasterol also protected against syringomycin, but they were less effective than cholesterol. The protective effect of sterols against the action of syringomycin is consistent with our hypothesis that membrane ergosterol is a critical component for syringomycin-binding as suggested by recent genetic studies.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-10-2323
1993-10-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/10/mic-139-10-2323.html?itemId=/content/journal/micro/10.1099/00221287-139-10-2323&mimeType=html&fmt=ahah

References

  1. BESSON F., PEYPOUX F., MICHEL G., DELCAMBE L. 1979; Antifungal activity upon Saccharomyces cerevisiae of iturin A, mycosubtilin, bacillomycin and of their derivatives; inhibition of this antifungal activity by lipid antagonists.. Journal of Antibiotics 32:828–833
    [Google Scholar]
  2. BIDWAI A.P., TAKEMOTO J.Y. 1987; Bacterial phytotoxin syringomycin, induces a protein kinase-mediated phosphorylation of red beet plasma membrane polypeptides.. Proceedings of the National Academy of Sciences of the United States of America 846755–6759
    [Google Scholar]
  3. DEVAY J.E., LUKEZIC F.L., SINDEN S.L., ENGLISH H., COPLIN D.L. 1968; A biocide produced by pathogenic isolates of Pseudomonas syringae and its possible role in the bacterial canker disease of peach trees.. Phytopathology 58:95–101
    [Google Scholar]
  4. GROSS D.C., DEVAY J.E. 1977; Role of syringomycin in holcus spot of maize and systemic necrosis of cowpea caused by Pseudomonas syringae.. Physiological Plant Pathology 11:1–11
    [Google Scholar]
  5. GROSS D.C., DEVAY J.E., STADMAN F. 1977; Chemical properties of syringomycin and syringotoxin; toxigenic peptides produced by Pseudomonas syringae.. Journal of Applied Bacteriology 43:453–463
    [Google Scholar]
  6. LAMPEN J.O. 1966; Interference by polyenic antifungal antibiotics (especially nystatin and filipin) with specific membrane functions.. Symposium of the Society for General Microbiology 16:111–130
    [Google Scholar]
  7. LAMPEN J.O., ARNOW P.M., BOROWSKA A., LASKIN A.I. 1962; Location and role of sterol at nystatin-binding sites.. Journal of Bacteriology 84:1152–1160
    [Google Scholar]
  8. LATOUD C., PEYPOUX F., MICHEL G. 1990; Interaction of iturin A, a lipopeptide antibiotic, with Saccharomyces cerevisiae cells: influence of the sterol membrane composition.. Canadian Journal of Microbiology 36:384–389
    [Google Scholar]
  9. LONGLEY R.P., ROSE A.H., KNIGHTS B.A. 1968; Composition of the protoplast membrane from Saccharomyces cerevisiae.. Bio-chemical Journal 108:401–412
    [Google Scholar]
  10. MARINI F., ARNOW P., LAMPEN J.O. 1961; The effect of monovalent cations on the inhibition of yeast metabolism by nystatin.. Journal of General Microbiology 24:51–62
    [Google Scholar]
  11. REIDL H.H., TAKEMOTO J.Y. 1987; Mechanism of action of bacterial phytotoxin, syringomycin. Simultaneous measurement of early responses in yeast and maize.. Biochimica et Biophysica Acta 898:59–69
    [Google Scholar]
  12. SINDEN S.L., DEVAY J.E., BACKMAN P.A. 1971; Properties of syringomycin, a wide spectrum antibiotic and phytotoxin produced by Pseudomonas syringae, and its role in the bacterial canker disease of peach trees.. Physiological Plant Pathology 1:199–213
    [Google Scholar]
  13. TAKEMOTO J.Y., ZHANG L., TAGUCHI N., TACHIKAWA T., MIYAKAWA T. 1991; Mechanism of action of the phytotoxin syringomycin: a resistant mutant of Saccharomyces cerevisiae reveals an involvement of Ca2+ transport.. Journal of General Microbiology 137:653–659
    [Google Scholar]
  14. TANAKA K., MATSUMOTO K., TOH-E A. 1988; Dual regulation of the expression of the polyubiquitin gene by cyclic AMP and heat shock in yeast.. EMBO Journal 7:495–502
    [Google Scholar]
  15. ZHANG L., TAKEMOTO J.Y. 1986; Mechanism of action of Pseudomonas syringae phytotoxin, syringomycin. Interaction with the plasma membrane of wild-type and respiratory-deficient strains of Saccharomyces cerevisiae.. Biochimica et Biophysica Acta 861:201–204
    [Google Scholar]
  16. ZHANG L., TAKEMOTO J.Y. 1987; Effect of Pseudomonas syringae phytotoxin, syringomycin, on plasma membrane fraction of Rhodotorula pilimanae.. Phytopathology 77:297–303
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-10-2323
Loading
/content/journal/micro/10.1099/00221287-139-10-2323
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error